rdf:type |
|
lifeskim:mentions |
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0031164,
umls-concept:C0033634,
umls-concept:C0035331,
umls-concept:C0205410,
umls-concept:C0225336,
umls-concept:C0376854,
umls-concept:C0755105,
umls-concept:C1280500,
umls-concept:C1555029
|
pubmed:issue |
1
|
pubmed:dateCreated |
2010-1-5
|
pubmed:abstractText |
Pathogenesis of diabetic macular edema is driven by deregulated expression of VEGF. A study of long-term exposure of immortalized bovine retinal endothelial cells (iBRECs) to VEGF(165) clearly confirmed the role of the tight junction protein claudin-1, which almost completely disappeared within 24 hours, an effect that was completely reversed by addition of the VEGF-binding Fab fragment ranibizumab. This study was conducted to investigate whether the VEGF(165)-induced loss of claudin-1 is regulated by protein kinase C (PKC) and indeed affects the barrier function of iBRECs.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
1552-5783
|
pubmed:author |
|
pubmed:issnType |
Electronic
|
pubmed:volume |
51
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
535-42
|
pubmed:meshHeading |
pubmed-meshheading:19643968-Animals,
pubmed-meshheading:19643968-Blotting, Western,
pubmed-meshheading:19643968-Capillary Permeability,
pubmed-meshheading:19643968-Cattle,
pubmed-meshheading:19643968-Cell Count,
pubmed-meshheading:19643968-Cells, Cultured,
pubmed-meshheading:19643968-Electric Impedance,
pubmed-meshheading:19643968-Endothelium, Vascular,
pubmed-meshheading:19643968-Membrane Proteins,
pubmed-meshheading:19643968-Microscopy, Fluorescence,
pubmed-meshheading:19643968-Protein Kinase C,
pubmed-meshheading:19643968-Protein Kinase Inhibitors,
pubmed-meshheading:19643968-Retinal Vessels,
pubmed-meshheading:19643968-Tetradecanoylphorbol Acetate,
pubmed-meshheading:19643968-Vascular Endothelial Growth Factor A
|
pubmed:year |
2010
|
pubmed:articleTitle |
Inhibition of protein kinase C is not sufficient to prevent or reverse effects of VEGF165 on claudin-1 and permeability in microvascular retinal endothelial cells.
|
pubmed:affiliation |
Department of Ophthalmology, University of Ulm Medical School, Ulm, Germany. heidrun.deissler@uniklinik-ulm.de
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|