Source:http://linkedlifedata.com/resource/pubmed/id/19643081
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2009-8-18
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pubmed:abstractText |
Inefficient cardiomyocyte differentiation limits the therapeutic use of embryonic stem (ES) cell-derived cardiomyocytes. While large collections of proprietary chemicals had been screened to improve ES cell differentiation into cardiomyocytes, the natural product library remained unexplored. Using a mouse ES cell line transfected with a cardiomyocyte-specific alpha-myosin heavy chain promoter-driven enhanced green fluorescent protein (EGFP) reporter, we screened 24 natural products with known cardioprotective actions. Salvianolic acid B (saB), while produced minimal effect on its own, concentration-dependently synergized with vitamin C in inducing cardiomyocyte differentiation, as demonstrated by an increase in EGFP(+) cells, beating area in embryoid bodies, and expression of cardiomyocyte maturity markers. This synergy is specific to cardiomyocyte differentiation, and is involved with collagen synthesis. The present study demonstrates the saB-vitamin C synergy in inducing ES cell differentiation into matured and functional cardiomyocytes, and this may lead to a practicable cocktail approach to generate ES cell-derived cardiomyocytes for cardiac stem cell therapy.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ascorbic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Benzofurans,
http://linkedlifedata.com/resource/pubmed/chemical/Cardiotonic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Vitamins,
http://linkedlifedata.com/resource/pubmed/chemical/salvianolic acid B
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1090-2104
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
2
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pubmed:volume |
387
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
723-8
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pubmed:meshHeading |
pubmed-meshheading:19643081-Animals,
pubmed-meshheading:19643081-Ascorbic Acid,
pubmed-meshheading:19643081-Benzofurans,
pubmed-meshheading:19643081-Cardiotonic Agents,
pubmed-meshheading:19643081-Cell Differentiation,
pubmed-meshheading:19643081-Cell Line,
pubmed-meshheading:19643081-Collagen,
pubmed-meshheading:19643081-Drug Evaluation, Preclinical,
pubmed-meshheading:19643081-Drug Synergism,
pubmed-meshheading:19643081-Embryonic Stem Cells,
pubmed-meshheading:19643081-Mice,
pubmed-meshheading:19643081-Myocytes, Cardiac,
pubmed-meshheading:19643081-Vitamins
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pubmed:year |
2009
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pubmed:articleTitle |
Salvianolic acid B-vitamin C synergy in cardiac differentiation from embryonic stem cells.
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pubmed:affiliation |
Institute of Clinical Medicine and Research Center for Clinical Medicine, National Cheng Kung University & Hospital, Tainan, Taiwan, ROC.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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