Linked Life Data

19641375

Source:http://linkedlifedata.com/resource/pubmed/id/19641375

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2009-11-30
pubmed:abstractText
Tumor-associated microtubule-associated protein (TMAP), also known as cytoskeleton associated protein 2 (CKAP2), has been recently shown to be involved in the assembly and maintenance of mitotic spindle and also plays an essential role in maintaining the fidelity of chromosome segregation during mitosis. We have previously reported that TMAP is phosphorylated at multiple residues specifically during mitosis, and characterized the mechanism and functional importance of phosphorylation at one of the mitosis-specific phosphorylation residues (i.e., Thr-622). However, the phosphorylation events at the remaining mitotic phosphorylation sites of TMAP have not been fully characterized in detail. Here, we report on generation and characterization of phosphorylated Thr-578- and phosphorylated Thr-596-specific antibodies. Using the antibodies, we show that phosphorylation of TMAP at Thr-578 and Thr-596 indeed occurs specifically during mitosis. Immunofluorescent staining using the antibodies shows that these residues become phosphorylated starting at prophase and then become rapidly dephosphorylated soon after initiation of anaphase. Subtle differences in the kinetics of phosphorylation between Thr-578 and Thr-596 imply that they may be under different mechanisms of phosphorylation during mitosis. Unlike the phosphorylation-deficient mutant form for Thr-622, the mutant in which both Thr-578 and Thr-596 had been mutated to alanines did not induce significant delay in progression of mitosis. These results show that the majority of mitosis-specific phosphorylation of TMAP is limited to pre-anaphase stages and suggest that the multiple phosphorylation may not act in concert but serve diverse functions.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-10559878, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-11149944, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-12058064, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-12727876, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-12942315, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-15282614, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-15504249, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-16876122, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-17339342, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-18779650, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-19158495, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-19369249, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-8548803, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-9362543, http://linkedlifedata.com/resource/pubmed/commentcorrection/19641375-9771967
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1226-3613
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
832-40
pubmed:dateRevised
2010-9-27
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Characterization of mitosis-specific phosphorylation of tumor-associated microtubule-associated protein.
pubmed:affiliation
Department of Molecular Cell Biology and Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-769, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't