Linked Life Data

19640148

Source:http://linkedlifedata.com/resource/pubmed/id/19640148

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2009-7-30
pubmed:abstractText
Gold nanoparticles have been found to greatly enhance the polymerase chain reaction (PCR) specificity and yield in recent studies. However, the underlying mechanism is still unclear, though different hypotheses have already been proposed. In this study, a mass-action based model has been developed to investigate the effect of Au nanoparticles on the two-round PCR results. The great affinity of Au nanoparticles to the single-stranded DNA is taken into consideration. Each nanoparticle is treated as a bioreactor and/or a selector, around which, reaction equations are coupled to simulate the particle effect, and to investigate the key parameters that might influence such an effect. It is assumed that there exists a competing mechanism between the specific and nonspecific bindings, both in the solution and on the particle surface during the reactions. The numerical predictions accord well to the experimental results, and can be used to explain the Au nanoparticles' effect on the enhancement of the PCR specificity and efficiency.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0148-0731
pubmed:author
pubmed:issnType
Print
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
074512
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Numerical simulation of Au nanoparticles effect on the PCR process.
pubmed:affiliation
Department of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, P.R. China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't