Source:http://linkedlifedata.com/resource/pubmed/id/19639585
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
14
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pubmed:dateCreated |
2009-8-3
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pubmed:abstractText |
Among the integrative gene therapy vectors developed to date, human immunodeficiency virus type 1 (HIV-1)-derived lentiviral vectors (LV) are distinguished by their capacity to infect both dividing and non-dividing cells. Recombinant LV particles contain viral proteins necessary for their packaging, infectious and integrating functions. Like the parental HIV-1 virus they are able to acquire various cellular proteins, but the number and localisation of these proteins are poorly characterised. In the present study we used 2-DE followed by MALDI-TOF to quantify the protein content of several types of vesicular stomatitis virus G-pseudotyped LV including those that were extensively purified in the perspective of clinical gene therapy studies. A proteinase K treatment was used to distinguish between cellular proteins incorporated into virions (I-proteins) and those co-purified with vectors (C-proteins). We found 10 C-proteins and 18 I-proteins associated with LV. Copy numbers for these core I-proteins varied from 5 (AIP-1/ALIX) to 280 (Cyclophilin A) per vector particle. Three novel I-proteins, guanine nucleotide-binding protein 2, L-lactate dehydrogenase B chain and hnRNP core protein A1, were found. This study defines for the first time, the protein stoichiometry of infectious HIV-1-derived LV particles.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1615-9861
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pubmed:author |
pubmed-author:CohenB DBD,
pubmed-author:DanosOlivierO,
pubmed-author:DenardJérômeJ,
pubmed-author:GalyAnneA,
pubmed-author:LaroudieNicolasN,
pubmed-author:MertenOtto-WilhelmOW,
pubmed-author:NaldiniLuigiL,
pubmed-author:RadrizzaniMarinaM,
pubmed-author:RundwasserStéphanieS,
pubmed-author:SvinartchoukFedorF
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pubmed:issnType |
Electronic
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3666-76
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pubmed:meshHeading |
pubmed-meshheading:19639585-Cell Line,
pubmed-meshheading:19639585-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:19639585-Genetic Vectors,
pubmed-meshheading:19639585-Humans,
pubmed-meshheading:19639585-Lentivirus,
pubmed-meshheading:19639585-Proteins,
pubmed-meshheading:19639585-Proteomics,
pubmed-meshheading:19639585-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:19639585-Viral Proteins
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pubmed:year |
2009
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pubmed:articleTitle |
Quantitative proteomic analysis of lentiviral vectors using 2-DE.
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pubmed:affiliation |
GENETHON, 91002 Evry, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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