Linked Life Data

1963275

Source:http://linkedlifedata.com/resource/pubmed/id/1963275

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-4-2
pubmed:abstractText
A procedure is described utilizing Percoll density media for the separation of nuclei and myofibrils in homogenates of adult skeletal muscle. Using this method, transcriptionally active nuclei can be readily obtained in relatively high yield (approximately 30%). In vitro RNA polymerase run-on-labeled RNAs isolated from these nuclei can be used in hybridization assays to study the transcriptional activities of specific genes. Percoll density gradient centrifugation should be useful for the isolation of nuclei from a variety of other tissues in which, like skeletal muscle, subcellular or tissue components cosediment with nuclei in conventional sucrose density centrifugation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
190
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
193-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Isolation of transcriptionally active nuclei from striated muscle using Percoll density gradients.
pubmed:affiliation
Department of Physiology and Neurobiology, University of Connecticut, Storrs 06269.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't