Source:http://linkedlifedata.com/resource/pubmed/id/19631747
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2009-9-11
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| pubmed:abstractText |
Matrix metalloproteinases (MMPs) are a large family of proteolytic enzymes involved in inflammation, wound healing and other pathological processes after neurological disorders. MMP-2 promotes functional recovery after spinal cord injury (SCI) by regulating the formation of a glial scar. In the present study, we aimed to investigate the expression and/or activity of several MMPs, after SCI and human umbilical cord blood mesenchymal stem cell (hUCB) treatment in rats with a special emphasis on MMP-2. Treatment with hUCB after SCI altered the expression of several MMPs in rats. MMP-2 is upregulated after hUCB treatment in spinal cord injured rats and in spinal neurons injured either with staurosporine or hydrogen peroxide. Further, hUCB induced upregulation of MMP-2 reduced formation of the glial scar at the site of injury along with reduced immunoreactivity to chondroitin sulfate proteoglycans. Blockade of MMP-2 activity in hUCB cocultured injured spinal neurons reduced the protection offered by hUCB which indicated the involvement of MMP-2 in the neuroprotection offered by hUCB. Based on these results, we conclude that hUCB treatment after SCI upregulates MMP-2 levels and reduces the formation of the glial scar thereby creating an environment suitable for endogenous repair mechanisms.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
|
| pubmed:issn |
1095-953X
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
36
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
200-12
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| pubmed:meshHeading |
pubmed-meshheading:19631747-Analysis of Variance,
pubmed-meshheading:19631747-Animals,
pubmed-meshheading:19631747-Animals, Newborn,
pubmed-meshheading:19631747-Cell Survival,
pubmed-meshheading:19631747-Cells, Cultured,
pubmed-meshheading:19631747-Cicatrix,
pubmed-meshheading:19631747-Coculture Techniques,
pubmed-meshheading:19631747-Cord Blood Stem Cell Transplantation,
pubmed-meshheading:19631747-Disease Models, Animal,
pubmed-meshheading:19631747-Fetal Blood,
pubmed-meshheading:19631747-Humans,
pubmed-meshheading:19631747-L-Lactate Dehydrogenase,
pubmed-meshheading:19631747-Male,
pubmed-meshheading:19631747-Matrix Metalloproteinase 2,
pubmed-meshheading:19631747-Neurons,
pubmed-meshheading:19631747-Rats,
pubmed-meshheading:19631747-Rats, Inbred Lew,
pubmed-meshheading:19631747-Rats, Sprague-Dawley,
pubmed-meshheading:19631747-Spinal Cord,
pubmed-meshheading:19631747-Spinal Cord Injuries,
pubmed-meshheading:19631747-Time Factors,
pubmed-meshheading:19631747-Tissue Inhibitor of Metalloproteinases,
pubmed-meshheading:19631747-Up-Regulation
|
| pubmed:year |
2009
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| pubmed:articleTitle |
Human umbilical cord blood stem cells upregulate matrix metalloproteinase-2 in rats after spinal cord injury.
|
| pubmed:affiliation |
Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|