Source:http://linkedlifedata.com/resource/pubmed/id/19631380
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2009-8-11
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| pubmed:abstractText |
In the cochlea, cell damage triggers intercellular Ca2+ waves that propagate through the glial-like supporting cells that surround receptor hair cells. These Ca2+ waves are thought to convey information about sensory hair cell-damage to the surrounding supporting cells within the cochlear epithelium. Mitochondria are key regulators of cytoplasmic Ca2+ concentration ([Ca2+](cyt)), and yet little is known about their role during the propagation of such intercellular Ca2+ signalling. Using neonatal rat cochlear explants and fluorescence imaging techniques, we explore how mitochondria modulate supporting cell [Ca2+](cyt) signals that are triggered by ATP or by hair cell damage. ATP application (0.1-50 microM) caused a dose dependent increase in [Ca2+](cyt) which was accompanied by an increase in mitochondrial calcium. Blocking mitochondrial Ca2+ uptake by dissipating the mitochondrial membrane potential using CCCP and oligomycin or using Ru360, an inhibitor of the mitochondrial Ca2+ uniporter, enhanced the peak amplitude and duration of ATP-induced [Ca2+](cyt) transients. In the presence of Ru360, the mean propagation velocity, amplitude and extent of spread of damage-induced intercellular Ca2+ waves was significantly increased. Thus, mitochondria function as spatial Ca2+ buffers during agonist-evoked [Ca2+](cyt) signalling in cochlear supporting cells and play a significant role in regulating the spatio-temporal properties of intercellular Ca2+ waves.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Carbonyl Cyanide m-Chlorophenyl...,
http://linkedlifedata.com/resource/pubmed/chemical/Ionophores,
http://linkedlifedata.com/resource/pubmed/chemical/Oligomycins,
http://linkedlifedata.com/resource/pubmed/chemical/Ru 360,
http://linkedlifedata.com/resource/pubmed/chemical/Ruthenium Compounds
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
1532-1991
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
46
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
136-46
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| pubmed:meshHeading |
pubmed-meshheading:19631380-Adenosine Triphosphate,
pubmed-meshheading:19631380-Animals,
pubmed-meshheading:19631380-Calcium,
pubmed-meshheading:19631380-Calcium Signaling,
pubmed-meshheading:19631380-Carbonyl Cyanide m-Chlorophenyl Hydrazone,
pubmed-meshheading:19631380-Cell Communication,
pubmed-meshheading:19631380-Cell Death,
pubmed-meshheading:19631380-Cells, Cultured,
pubmed-meshheading:19631380-Cochlea,
pubmed-meshheading:19631380-Hair Cells, Auditory,
pubmed-meshheading:19631380-Ion Transport,
pubmed-meshheading:19631380-Ionophores,
pubmed-meshheading:19631380-Labyrinth Supporting Cells,
pubmed-meshheading:19631380-Membrane Potential, Mitochondrial,
pubmed-meshheading:19631380-Mitochondria,
pubmed-meshheading:19631380-Oligomycins,
pubmed-meshheading:19631380-Rats,
pubmed-meshheading:19631380-Rats, Sprague-Dawley,
pubmed-meshheading:19631380-Ruthenium Compounds
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| pubmed:year |
2009
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| pubmed:articleTitle |
Mitochondria modulate the spatio-temporal properties of intra- and intercellular Ca2+ signals in cochlear supporting cells.
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| pubmed:affiliation |
UCL Ear Institute, 332 Gray's Inn Road, London WC1X 8EE, UK; Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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