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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2009-7-27
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pubmed:abstractText |
Death-associated protein kinase (DAPK) is a serine/threonine kinase that contributes to pro-apoptotic signaling on cytokine exposure. The role of DAPK in macrophage-associated tumor cell death is currently unknown. Recently, we suggested a new function for DAPK in the induction of apoptosis during the interaction between colorectal tumor cells and tumor-associated macrophages. Using a cell-culture model with conditioned supernatants of differentiated/activated macrophages (U937) and human HCT116 colorectal tumor cells, we replicated DAPK-associated tumor cell death; this model likely reflects the in vivo tumor setting. In this study, we show that tumor necrosis factor-alpha exposure under conditions of macrophage activation induced DAPK-dependent apoptosis in the colorectal tumor cell line HCT116. Simultaneously, early phosphorylation of p38 mitogen-activated protein kinase (phospho-p38) was observed. We identified the phospho-p38 mitogen-activated protein kinase as a novel interacting protein of DAPK in tumor necrosis factor-alpha-induced apoptosis. The general relevance of this interaction was verified in two colorectal cell lines without functional p53 (ie, HCT116 p53(-/-) and HT29 mutant) and in human colon cancer and ulcerative colitis tissues. Supernatants of freshly isolated human macrophages were also able to induce DAPK and phospho-p38. Our findings highlight the mechanisms that underlie DAPK regulation in tumor cell death evoked by immune cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-10402466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-10411570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-10669763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-11146619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-11229684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-11729103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-15187187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-15608685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-16164034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-16575786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-17087515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-7828849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-8662985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-8675585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-8690407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-9525929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-9553074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19628771-9819420
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1525-2191
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pubmed:author |
pubmed-author:BajboujKhuloudK,
pubmed-author:BommhardtUrsulaU,
pubmed-author:ChakilamSarithaS,
pubmed-author:DrewesThomasT,
pubmed-author:HaaseKathrinK,
pubmed-author:HartigRolandR,
pubmed-author:IvanovskaJelenaJ,
pubmed-author:KlicheStefanieS,
pubmed-author:KuesterDoertheD,
pubmed-author:LeverkusMartinM,
pubmed-author:PoehlmannAngelaA,
pubmed-author:RoessnerAlbertA,
pubmed-author:Schneider-StockRegineR,
pubmed-author:TellerAnneA,
pubmed-author:UlitzschAnnikaA,
pubmed-author:WalluscheckDianaD
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pubmed:issnType |
Electronic
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pubmed:volume |
175
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
557-70
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pubmed:dateRevised |
2010-9-27
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pubmed:meshHeading |
pubmed-meshheading:19628771-Apoptosis,
pubmed-meshheading:19628771-Apoptosis Regulatory Proteins,
pubmed-meshheading:19628771-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:19628771-Cell Line, Tumor,
pubmed-meshheading:19628771-Colorectal Neoplasms,
pubmed-meshheading:19628771-Humans,
pubmed-meshheading:19628771-Immunoprecipitation,
pubmed-meshheading:19628771-Interferon-gamma,
pubmed-meshheading:19628771-Macrophage Activation,
pubmed-meshheading:19628771-Macrophages,
pubmed-meshheading:19628771-Phosphorylation,
pubmed-meshheading:19628771-Tumor Necrosis Factor-alpha,
pubmed-meshheading:19628771-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2009
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pubmed:articleTitle |
Identification of phosphorylated p38 as a novel DAPK-interacting partner during TNFalpha-induced apoptosis in colorectal tumor cells.
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pubmed:affiliation |
Experimental Tumor Pathology, Institute of Pathology, University of Erlangen-Nuremberg, Universitätsstr. 22, 91054 Erlangen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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