Linked Life Data

19625175

Source:http://linkedlifedata.com/resource/pubmed/id/19625175

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-10-5
pubmed:abstractText
We have previously demonstrated that the EP1 subtype of PGE2 receptor is expressed in the differentiated compartment of normal human epidermis and is coupled to intracellular calcium mobilization. We therefore hypothesized that the EP1 receptor is coupled to keratinocyte differentiation. In in vitro studies, radioligand binding, RT-PCR, immunoblot and receptor agonist-induced second messenger studies demonstrate that the EP1 receptor is up-regulated by high cell density in human keratinocytes and this up-regulation precedes corneocyte formation. Moreover, two different EP1 receptor antagonists, SC51322 and AH6809, both inhibited corneocyte formation. SC51322 also inhibited the induction of differentiation-specific proteins, cytokeratin K10 and epidermal transglutaminase. We next examined the immunolocalization of the EP1 receptor in non-melanoma skin cancer in humans. Well-differentiated SCCs exhibited significantly greater membrane staining, while spindle cell carcinomas and BCCs had significantly decreased membrane staining compared with normal epidermis. This data supports a role for the EP1 receptor in regulating keratinocyte differentiation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1532-2823
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
279-90
pubmed:dateRevised
2011-6-20
pubmed:meshHeading
pubmed-meshheading:19625175-Animals, pubmed-meshheading:19625175-Calcium, pubmed-meshheading:19625175-Cell Differentiation, pubmed-meshheading:19625175-Humans, pubmed-meshheading:19625175-Immunohistochemistry, pubmed-meshheading:19625175-Keratinocytes, pubmed-meshheading:19625175-Mice, pubmed-meshheading:19625175-Mice, Inbred C57BL, pubmed-meshheading:19625175-Mice, Knockout, pubmed-meshheading:19625175-Phenotype, pubmed-meshheading:19625175-Receptors, Prostaglandin E, pubmed-meshheading:19625175-Receptors, Prostaglandin E, EP1 Subtype, pubmed-meshheading:19625175-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19625175-Signal Transduction, pubmed-meshheading:19625175-Skin Neoplasms, pubmed-meshheading:19625175-Tumor Cells, Cultured, pubmed-meshheading:19625175-Xanthones
pubmed:year
2009
pubmed:articleTitle
The EP1 subtype of prostaglandin E2 receptor: role in keratinocyte differentiation and expression in non-melanoma skin cancer.
pubmed:affiliation
Department of Pathology & Laboratory Medicine, Indiana University School of Medicine, 1120 South Drive, Fesler Hall 403, Indianapolis, IN 46202, USA. rkonger@iupui.edu
pubmed:publicationType
Journal Article