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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1992-1-9
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pubmed:abstractText |
Immunoglobulin and T-cell receptor genes are somatically rearranged by site-specific recombination. Recombination signal sequences (RSS) have been identified as the major targeting element of this process. Recent reports demonstrate that differences in RSS affect the frequency of recombination, suggesting a role for RSS in the development of the B-cell repertoire. Examination of mouse light-chain RSS indicates that kappa light-chain RSS consistently show a greater degree of similarity to a consensus sequence than do those of lambda light chain. To determine whether this difference in natural RSS could affect the patterns of light-chain gene rearrangement and expression, we have constructed recombination substrates containing both a typical mouse kappa RSS pair and a typical mouse lambda RSS pair. Experiments using these substrates demonstrate that the kappa RSS pair mediates recombination at a vastly higher frequency than does the lambda RSS pair. This result argues that RSS differences may contribute significantly to the patterns of mouse immunoglobulin light-chain rearrangement, ultimately resulting in a high proportion of kappa light chain relative to lambda.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-111144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-14169351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-1900459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-1901886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-1901887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-1911542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-2105884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-2115572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-2136205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-2141629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-2153073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-2493585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-2505932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-2548157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-2777075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-2847166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-3018508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-3035056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-3041370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-3081897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-3110779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-3120312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-3139764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-3428598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-3495343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-6277505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-6291785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-6300689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-6405281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-6438633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-6769117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-6783958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-6783959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-6817330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-6818320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1961738-7024412
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10721-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1961738-Animals,
pubmed-meshheading:1961738-Base Sequence,
pubmed-meshheading:1961738-Cell Line,
pubmed-meshheading:1961738-DNA Nucleotidyltransferases,
pubmed-meshheading:1961738-Gene Rearrangement,
pubmed-meshheading:1961738-Immunoglobulin Light Chains,
pubmed-meshheading:1961738-Immunoglobulin kappa-Chains,
pubmed-meshheading:1961738-Immunoglobulin lambda-Chains,
pubmed-meshheading:1961738-Mice,
pubmed-meshheading:1961738-Molecular Sequence Data,
pubmed-meshheading:1961738-Oligodeoxyribonucleotides,
pubmed-meshheading:1961738-Plasmids,
pubmed-meshheading:1961738-Protein Sorting Signals,
pubmed-meshheading:1961738-Recombination, Genetic,
pubmed-meshheading:1961738-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1961738-VDJ Recombinases
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pubmed:year |
1991
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pubmed:articleTitle |
Mouse kappa light-chain recombination signal sequences mediate recombination more frequently than do those of lambda light chain.
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pubmed:affiliation |
Department of Immunology, University of Toronto, Ontario, Canada.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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