Linked Life Data

19616003

Source:http://linkedlifedata.com/resource/pubmed/id/19616003

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-9-7
pubmed:abstractText
Clinical evidence indicates that intensive insulin therapy during critical illness protects the endothelium and contributes to prevention of organ failure and death but the mechanisms involved remain unclear. This study was designed to test the hypothesis that insulin inhibits adherence of polymorphonuclear leukocytes (PMNs) to endothelial cells in myocardial ischemia/reperfusion (MI/R) and to investigate the underlying mechanisms. Anesthetized rabbits were subjected to MI/R (45 min/4 h) and randomly received saline, glucose-insulin-potassium (GIK) or GK respectively (2 mL/kg/h, i.v.). In vitro study was performed on cultured endothelial cells subjected to simulated ischemia/reperfusion. In vivo treatment with GIK but not GK attenuated myocardial injury as evidenced by reduced plasma creatine kinase activity, myocardial apoptosis and infarct size in MI/R rabbits compared with the saline group. Interestingly, GIK but not GK significantly decreased coronary endothelial expression of P-selectin and intercellular adhesion molecule-1 (ICAM-1), inhibited adherence of PMNs to coronary endothelium (107.7+/-7.4 vs. 155.0+/-9.2 PMNs/mm(2) in saline group, n=8, P<0.01), and therefore decreased myocardial PMNs accumulation. In cultured endothelial cells subjected to simulated ischemia/reperfusion, insulin (10(-)(7) M) increased Akt activity and eNOS phosphorylation with subsequent NO production, and concurrently exerted an anti-adhesive effect as manifested by reduced endothelial P-selectin and ICAM-1 surface expression and PMNs adherence (13.7+/-1.3% vs. 22.2+/-1.9% in vehicle, n=9, P<0.01), all of which are abolished by the specific Akt inhibitor. Furthermore, inhibition of insulin-stimulated NO production using either the selective eNOS inhibitor cavtratin or the NOS inhibitor L-NAME blocked the anti-adhesive effect of insulin. These results demonstrate that insulin reduces endothelial P-selectin and ICAM-1 expression, and thus inhibits leukocyte-endothelium adherence in MI/R rabbit hearts. The anti-adhesive property by insulin may be mediated by the Akt-mediated and NO-dependent pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1095-8584
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
512-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:19616003-Animals, pubmed-meshheading:19616003-Blood Glucose, pubmed-meshheading:19616003-Cell Adhesion, pubmed-meshheading:19616003-Cell Membrane, pubmed-meshheading:19616003-Endothelial Cells, pubmed-meshheading:19616003-Enzyme Activation, pubmed-meshheading:19616003-Glycogen Synthase Kinase 3, pubmed-meshheading:19616003-Humans, pubmed-meshheading:19616003-Insulin, pubmed-meshheading:19616003-Intercellular Adhesion Molecule-1, pubmed-meshheading:19616003-Myocardial Reperfusion Injury, pubmed-meshheading:19616003-Neutrophils, pubmed-meshheading:19616003-Nitric Oxide, pubmed-meshheading:19616003-Nitric Oxide Synthase Type III, pubmed-meshheading:19616003-P-Selectin, pubmed-meshheading:19616003-Phosphorylation, pubmed-meshheading:19616003-Proto-Oncogene Proteins c-akt, pubmed-meshheading:19616003-Rabbits
pubmed:year
2009
pubmed:articleTitle
Insulin inhibits leukocyte-endothelium adherence via an Akt-NO-dependent mechanism in myocardial ischemia/reperfusion.
pubmed:affiliation
Department of Physiology and Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't