|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0020964,
umls-concept:C0039195,
umls-concept:C0205314,
umls-concept:C0376315,
umls-concept:C0439851,
umls-concept:C0591833,
umls-concept:C0679622,
umls-concept:C1523987,
umls-concept:C1524095,
umls-concept:C1552596,
umls-concept:C1947931,
umls-concept:C2239176
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2009-9-21
|
|
pubmed:abstractText |
Mouse Hepa1-6 hepatocellular carcinoma (HCC) cells were transduced with the membrane form of macrophage colony stimulating factor (mM-CSF). When mM-CSF transduced Hepa1-6 cells were injected subcutaneously into mice, these cells did not form tumors. The spleens of these immunized mice contained cytotoxic CD8+ T lymphocytes (CTL) that killed the unmodified Hepa1-6 cells. We show that the alternative form of macrophage colony stimulating factor (altM-CSF) induced CTL-mediated immunity against Hepa1-6 cells. AltM-CSF is restricted to the H-2D(b) allele. CTLs killed RMA-S cells loaded with exogenous altM-CSF peptide. Vaccination of mice with dendritic cells pulsed with the altM-CSF peptide stimulated anti-Hepa1-6 CTLs. Hyper-immunization of mice with mM-CSF Hepa1-6 cells showed inflammation of the liver and kidneys. Although altM-CSF was expressed within liver and kidney cells, its intensity was lower than Hepa1-6 cells. AltM-CSF was detected within the human HepG2 cell line. These studies suggest that altM-CSF may be a tumor antigen for HCC.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
1090-2163
|
|
pubmed:author |
pubmed-author:AlipanahRezaR,
pubmed-author:BoussaidOO,
pubmed-author:DanQinghongQ,
pubmed-author:DelgadoChristinaC,
pubmed-author:GeLishengL,
pubmed-author:HoaNeilN,
pubmed-author:JadusMartin RMR,
pubmed-author:MorganTimothy RTR,
pubmed-author:NguyenTuong-ViTV,
pubmed-author:PhamJimmy T HJT,
pubmed-author:SamathanamChristina ACA,
pubmed-author:SanchezRamonR,
pubmed-author:Tarbiyat-BoldajiMaryM,
pubmed-author:ZhangJian GangJG
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
259
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
117-27
|
|
pubmed:meshHeading |
pubmed-meshheading:19615673-Animals,
pubmed-meshheading:19615673-Antigens, Neoplasm,
pubmed-meshheading:19615673-CD8-Positive T-Lymphocytes,
pubmed-meshheading:19615673-Cell Line, Tumor,
pubmed-meshheading:19615673-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:19615673-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:19615673-Flow Cytometry,
pubmed-meshheading:19615673-Humans,
pubmed-meshheading:19615673-Immunization,
pubmed-meshheading:19615673-Immunohistochemistry,
pubmed-meshheading:19615673-Liver Neoplasms, Experimental,
pubmed-meshheading:19615673-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:19615673-Mice,
pubmed-meshheading:19615673-Mice, Inbred C57BL,
pubmed-meshheading:19615673-Microscopy, Fluorescence,
pubmed-meshheading:19615673-Protein Isoforms,
pubmed-meshheading:19615673-RNA,
pubmed-meshheading:19615673-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19615673-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:19615673-Transduction, Genetic
|
|
pubmed:year |
2009
|
|
pubmed:articleTitle |
Cytotoxic T cell immunity against the non-immunogenic, murine, hepatocellular carcinoma Hepa1-6 is directed towards the novel alternative form of macrophage colony stimulating factor.
|
|
pubmed:affiliation |
Pathology and Laboratory Medicine Service, Diagnostic and Molecular Medicine Health Care Group, VA Long Beach Healthcare System, 5901 E. 7th Street, Long Beach, CA 90822, USA.
|
|
pubmed:publicationType |
Journal Article
|
