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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
33
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pubmed:dateCreated |
2009-8-18
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pubmed:abstractText |
Increases in the power stroke and dwell durations of single molecules of Escherichia coli F(1)-ATPase were measured in response to viscous loads applied to the motor and inhibition of ATP hydrolysis. The load was varied using different sizes of gold nanorods attached to the rotating gamma subunit and/or by increasing the viscosity of the medium using PEG-400, a noncompetitive inhibitor of ATPase activity. Conditions that increase the duration of the power stroke were found to cause 20-fold increases in the length of the dwell. These results suggest that the order of hydrolysis, product release, and substrate binding may change as the result of external load on the motor or inhibition of hydrolysis.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19610671-11062563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19610671-11309608,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19610671-9824301
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1520-4995
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
25
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7979-85
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pubmed:dateRevised |
2010-9-27
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pubmed:meshHeading |
pubmed-meshheading:19610671-Adenosine Triphosphate,
pubmed-meshheading:19610671-Catalysis,
pubmed-meshheading:19610671-Cell Membrane,
pubmed-meshheading:19610671-Enzyme Inhibitors,
pubmed-meshheading:19610671-Escherichia coli Proteins,
pubmed-meshheading:19610671-Hydrolysis,
pubmed-meshheading:19610671-Molecular Motor Proteins,
pubmed-meshheading:19610671-Polyethylene Glycols,
pubmed-meshheading:19610671-Protein Subunits,
pubmed-meshheading:19610671-Proton-Motive Force,
pubmed-meshheading:19610671-Proton-Translocating ATPases,
pubmed-meshheading:19610671-Time Factors,
pubmed-meshheading:19610671-Viscosity
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pubmed:year |
2009
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pubmed:articleTitle |
Single molecule measurements of F1-ATPase reveal an interdependence between the power stroke and the dwell duration.
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pubmed:affiliation |
School of Life Sciences, Arizona State University, P.O. Box 874501, Tempe, Arizona 85287-4501, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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