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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2009-9-2
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pubmed:abstractText |
A pyridyl disulfide end-functionalized polymer with N-acetyl-d-glucosamine pendant side-chains was synthesized by atom transfer radical polymerization (ATRP). The glycopolymer was prepared from a pyridyl disulfide initiator catalyzed by a Cu(I)/Cu(II)/2,2'-bipyridine system in a mixture of methanol and water at 30 degrees C. The final polymer had a number-average molecular weight (M(n)) of 13.0 kDa determined by (1)H NMR spectroscopy and a narrow polydispersity index (1.12) determined by gel permeation chromatography (GPC). The pyridyl disulfide end-group was then utilized to conjugate the glycopolymer to a double-stranded short interfering RNA (siRNA). Characterization of the glycopolymer-siRNA by polyacrylamide gel electrophoresis (PAGE) showed 97% conjugation. The activated disulfide polymer was also patterned on gold via microcontact printing. The pyridyl disulfide allowed for ready immobilization of the glycopolymer into 200 microm sized features on the surface.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19606855-10026133,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19606855-19206608
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1526-4602
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
10
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2207-12
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pubmed:dateRevised |
2010-9-27
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pubmed:meshHeading |
pubmed-meshheading:19606855-2,2'-Dipyridyl,
pubmed-meshheading:19606855-Biocompatible Materials,
pubmed-meshheading:19606855-Chromatography, Gel,
pubmed-meshheading:19606855-Disulfides,
pubmed-meshheading:19606855-Gold,
pubmed-meshheading:19606855-Magnetic Resonance Spectroscopy,
pubmed-meshheading:19606855-Polymers,
pubmed-meshheading:19606855-Polysaccharides,
pubmed-meshheading:19606855-RNA, Small Interfering,
pubmed-meshheading:19606855-Surface Properties
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pubmed:year |
2009
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pubmed:articleTitle |
Synthesis of a pyridyl disulfide end-functionalized glycopolymer for conjugation to biomolecules and patterning on gold surfaces.
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pubmed:affiliation |
Department of Chemistry and Biochemistry and California Nanosystems Institute, University of California-Los Angeles, Los Angeles, California 90095-1569.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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