Linked Life Data

19606426

Source:http://linkedlifedata.com/resource/pubmed/id/19606426

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2009-8-17
pubmed:abstractText
Previously, we investigated the antimicrobial properties of pleurocidin (Ple) enantiomers. Our studies showed that the L-enantiomer exhibited about a 2-16 fold more potent activity against bacterial strains as compared to that of the D-enantiomer. However, fungal strains were about two-fold more susceptible to the D-enantiomer than to the L-enantiomer. In this study, confocal laser scanning microscopy indicates that the Ple enantiomers internalize into the cell surface. The present results also suggest that they could be characterized by a membrane-active mechanism. To further elucidate their selective membranolytic activities, we conducted a fluorescence analysis. A study with 1,6-diphenyl-1,3,5-hexatriene, a hydrophobic molecule, showed that the L-and the D-enantiomer exert more potent antibacterial or antifungal activity than their opposite enantiomer, respectively. Furthermore, we synthesized liposomes by using representative phospholipids consisting of bacterial or fungal membranes. Our results show that the L-enantiomer causes significant dye leakage from negatively charged liposomes (PG/CL; 58:42, PC/PG; 1:1, w/w) which mimic bacterial membranes such as Staphylococcus aureus. Conversely, the D-enantiomer has more potent leakage effects against fungal liposomes (PC/PE/PI/ergosterol; 5:4:1:2, w/w/w/w, PC/ergosterol; 10:1, w/w). In summary, these results suggest that the selective antimicrobial effects of the Ple enantiomers against bacterial and fungal cells may be due to the different lipid compositions of prokaryotes and eukaryotes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1099-1387
pubmed:author
pubmed:copyrightInfo
Copyright 2009 European Peptide Society and John Wiley & Sons, Ltd.
pubmed:issnType
Electronic
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
601-6
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Cell selectivity-membrane phospholipids relationship of the antimicrobial effects shown by pleurocidin enantiomeric peptides.
pubmed:affiliation
School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu 702-701, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't