Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 8
pubmed:dateCreated
2009-8-5
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB360546, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB360547, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB468054, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB468055, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB468056, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB468057, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB468952, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB468953, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB468954, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB468955, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB469015, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB469016, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB469017, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB469018
pubmed:abstractText
Two Gram-variable, aerobic, motile, rod-shaped, endospore-forming bacterial strains, M4-2T and M4-1, were isolated from soil samples collected from Oblast Magadan, Russian Far East, as micro-organisms antagonistic to the psychrophilic phytopathogenic fungus Typhula ishikariensis. Strains M4-2T and M4-1 were identified as members of the genus Paenibacillus by phenotypic and phylogenetic analyses based on 16S rRNA gene sequences. The strains contained anteiso-C15:0 as the major fatty acid (63.0-64.7%) and MK-7 as the major isoprenoid quinone. The DNA G+C contents were 42.8 and 41.7 mol%, respectively. 16S rRNA gene sequence analysis showed that strains M4-2T and M4-1 exhibited high similarities with Paenibacillus macquariensis DSM 2T (99.5 and 99.7%, respectively) and Paenibacillus antarcticus LMG 22078T (99.4 and 99.5%, respectively). There were no clear differences in the phenotypic characteristics and chemotaxonomic and phylogenetic data between the novel isolates and P. macquariensis DSM 2T. DNA-DNA hybridization experiments between strain M4-2T and P. macquariensis DSM 2T and P. antarcticus LMG 22078T revealed reassociation values of 56 and 49%, respectively. Multilocus sequence analysis confirmed the differences between the new isolates and reference strains that were observed with the DNA-DNA hybridization studies. On the basis of the results described above, it is proposed that the isolates represent a novel subspecies of P. macquariensis, Paenibacillus macquariensis subsp. defensor subsp. nov. The type strain is M4-2T (=JCM 14954T=NCIMB 14397T).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1466-5026
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2074-9
pubmed:meshHeading
pubmed-meshheading:19605719-Aerobiosis, pubmed-meshheading:19605719-Antibiosis, pubmed-meshheading:19605719-Bacterial Typing Techniques, pubmed-meshheading:19605719-Base Composition, pubmed-meshheading:19605719-Basidiomycota, pubmed-meshheading:19605719-Cluster Analysis, pubmed-meshheading:19605719-DNA, Bacterial, pubmed-meshheading:19605719-DNA, Ribosomal, pubmed-meshheading:19605719-Far East, pubmed-meshheading:19605719-Fatty Acids, pubmed-meshheading:19605719-Gram-Positive Bacteria, pubmed-meshheading:19605719-Molecular Sequence Data, pubmed-meshheading:19605719-Nucleic Acid Hybridization, pubmed-meshheading:19605719-Phylogeny, pubmed-meshheading:19605719-Quinones, pubmed-meshheading:19605719-RNA, Ribosomal, 16S, pubmed-meshheading:19605719-Sequence Analysis, DNA, pubmed-meshheading:19605719-Soil Microbiology, pubmed-meshheading:19605719-Spores, Bacterial
pubmed:year
2009
pubmed:articleTitle
Paenibacillus macquariensis subsp. defensor subsp. nov., isolated from boreal soil.
pubmed:affiliation
Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo 062-8517, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't