Linked Life Data

19604096

Source:http://linkedlifedata.com/resource/pubmed/id/19604096

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2009-12-23
pubmed:abstractText
An important but poorly understood feature of traumatic brain injury (TBI) is the clinically serious problem of spatiotemporal progression ("blossoming") of a hemorrhagic contusion, a phenomenon we term progressive secondary hemorrhage (PSH). Molecular mechanisms of PSH are unknown and efforts to reduce it by promoting coagulation have met with equivocal results. We hypothesized that PSH might be due to upregulation and activation of sulfonylurea receptor 1 (SUR1)-regulated NC(Ca-ATP) channels in capillary endothelial cells, predisposing to oncotic death of endothelial cells and catastrophic failure of capillary integrity. Anesthetized adult male rats underwent left parietal craniectomy for induction of a focal cortical contusion. The regulatory subunit of the channel, SUR1, was prominently upregulated in capillaries of penumbral tissues surrounding the contusion. In untreated rats, PSH was characterized by progressive enlargement of the contusion deep into the site of cortical impact, including corpus callosum, hippocampus, and thalamus, by progressive accumulation of extravasated blood, with a doubling of the volume during the first 12 h after injury, and by capillary fragmentation in penumbral tissues. Block of SUR1 using low-dose (non-hypoglycemogenic) glibenclamide largely eliminated PSH and capillary fragmentation, and was associated with a significant reduction in the size of the necrotic lesion and in preservation of neurobehavioral function. Antisense oligodeoxynucleotide against SUR1, administered after injury, reduced both SUR1 expression and PSH, consistent with a requirement for transcriptional upregulation of SUR1. Our findings provide novel insights into molecular mechanisms responsible for PSH associated with hemorrhagic contusions, and point to SUR1 as a potential therapeutic target in TBI.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1557-9042
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2257-67
pubmed:dateRevised
2011-3-3
pubmed:meshHeading
pubmed-meshheading:19604096-ATP-Binding Cassette Transporters, pubmed-meshheading:19604096-Animals, pubmed-meshheading:19604096-Blood-Brain Barrier, pubmed-meshheading:19604096-Brain, pubmed-meshheading:19604096-Brain Hemorrhage, Traumatic, pubmed-meshheading:19604096-Brain Injuries, pubmed-meshheading:19604096-Calcium Channels, pubmed-meshheading:19604096-Disease Models, Animal, pubmed-meshheading:19604096-Down-Regulation, pubmed-meshheading:19604096-Endothelial Cells, pubmed-meshheading:19604096-Glyburide, pubmed-meshheading:19604096-Hypoglycemic Agents, pubmed-meshheading:19604096-Male, pubmed-meshheading:19604096-Neuroprotective Agents, pubmed-meshheading:19604096-Oligodeoxyribonucleotides, Antisense, pubmed-meshheading:19604096-Potassium Channels, Inwardly Rectifying, pubmed-meshheading:19604096-Rats, pubmed-meshheading:19604096-Rats, Long-Evans, pubmed-meshheading:19604096-Receptors, Drug, pubmed-meshheading:19604096-Up-Regulation
pubmed:year
2009
pubmed:articleTitle
Key role of sulfonylurea receptor 1 in progressive secondary hemorrhage after brain contusion.
pubmed:affiliation
Department of Neurosurgery, University of Maryland School of Medicine, Baltimore, Maryland, USA. msimard@smail.umaryland.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural