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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1977-9-22
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pubmed:abstractText |
An indirect solid-phase microradioimmunoassay is described for detecting antibodies against rotaviruses. The test involved ethanol fixation of microcultures of bovine rotavirus-infected BSC-1 cells and reacetion with bovine antirotavirus serum, followed by 125I-labeled rabbit anti-bovine immunoglobulin G. The technique was shown to be virus specific and highly sensitive. The fixed microcultures could be stored at 4 degrees C for at least 2 months without affecting the sensitivity of the test. The application of this system for the detection of rotavirus antibodies in humans is briefly discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-1270600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-163517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-170309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-172526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-178691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-4127639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-4136845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-4137164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-4623479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-4627513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-46592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-4972583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-5093775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-53206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-61589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-793991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/195982-965097
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6?-71
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:195982-Animals,
pubmed-meshheading:195982-Antibodies, Viral,
pubmed-meshheading:195982-Cattle,
pubmed-meshheading:195982-Diarrhea Viruses, Bovine Viral,
pubmed-meshheading:195982-Ethanol,
pubmed-meshheading:195982-Fixatives,
pubmed-meshheading:195982-Immunoglobulin G,
pubmed-meshheading:195982-Iodine Radioisotopes,
pubmed-meshheading:195982-Methanol,
pubmed-meshheading:195982-Methods,
pubmed-meshheading:195982-RNA Viruses,
pubmed-meshheading:195982-Radioimmunoassay,
pubmed-meshheading:195982-Reoviridae
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pubmed:year |
1977
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pubmed:articleTitle |
Solid-phase radioimmunoassay for detecting bovine (neonatal calf diarrhea) rotavirus antibody.
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pubmed:publicationType |
Journal Article
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