Linked Life Data

19597710

Source:http://linkedlifedata.com/resource/pubmed/id/19597710

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2011-4-6
pubmed:abstractText
Scedosporium apiospermum is an emerging pathogen colonizing the airways of patients with cystic fibrosis and causing severe infections in immunocompromised hosts. In order to improve our knowledge on the pathogenic mechanisms of this fungus, we investigated the production of siderophores. Cultivation on CAS medium and specific assays for different classes of siderophores suggested the secretion of hydroxamates. A maximal production was obtained by cultivation of the fungus at alkaline pH in an iron-restricted liquid culture medium. Siderophores were then extracted from the culture filtrate by liquid/liquid extraction, and separated by reverse phase high performance liquid chromatography. Two siderophores, dimerumic acid and N?-methyl coprogen B, were identified by electrospray ionization-mass spectrometry and MS-MS fragmentation. Finally, comparison of various strains suggested a higher production of Na-methyl coprogen B by clinical isolates of respiratory origin. Studies are initiated in order to determine the potential usefulness of these siderophores as diagnostic markers of scedosporiosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1572-8773
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1019-29
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Hydroxamate siderophores of Scedosporium apiospermum.
pubmed:affiliation
Laboratoire des Substances Naturelles et Analogues Structuraux, UPRES-EA 921, IFR 149 QUASAV, UFR de Pharmacie et Ingénierie de Santé, Université Université d'Angers,16 Bd Daviers, 49000 Angers, France
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't