Linked Life Data

19594391

Source:http://linkedlifedata.com/resource/pubmed/id/19594391

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-9-15
pubmed:abstractText
Recent findings identifying the transcription factors involved in the regulation of pluripotency and self-renewal in embryonic stem cells (ESC) may provide keys that enable the derivation of ESC in domestic species. In this study we monitored the expression of pluripotency-related genes in bovine inner cell mass (ICM) explants during the critical first steps in establishment of primary cultures. The expression of NANOG and POU5F1 transcripts and proteins in intact, in vitro produced (IVP) blastocysts was confirmed by quantitative RT-PCR and fluorescent immunocytochemistry. NANOG was localized to the nucleoplasm as well as the nucleoli in the ICM, whereas it appeared to be restricted to the nucleoli in trophectoderm cells. POU5F1 was localized in the nuclei of ICM and trophectoderm cells. ICM explants were analyzed by quantitative PCR and semiquantitative RT-PCR. The three major pluripotency-related transcription factors, NANOG, POU5F1, and SOX2, were expressed initially in the ICM explants, but were downregulated with subsequent culture. Markers of differentiation (BMP4, HNF4, NCAM, CDX2) and genes involved in LIF, BMP, and WNT signaling pathways were also expressed. ICM explants were cultured in the presence of various concentrations of cytokines belonging to the TGF-beta superfamily. Noggin, a cytokine inhibiting the BMP4 pathway, successfully upregulated the relative expression of NANOG mRNA in the ICM explants with respect to controls.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1557-7457
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
355-65
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Expression of pluripotency-related genes during bovine inner cell mass explant culture.
pubmed:affiliation
Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland 20742, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't