Linked Life Data

19591460

Source:http://linkedlifedata.com/resource/pubmed/id/19591460

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2009-9-2
pubmed:abstractText
The physiological roles of free Zn(2+) have attracted great attention. To clarify those roles, there has been a need for ratiometric fluorescent Zn(2+) probes for practical use. We report the rational design and synthesis of a series of ratiometric fluorescent Zn(2+) probes. The structures of the probes are based on the 7-hydroxycoumarin structure. We focused on the relationship between the electron-donating ability of the 7-hydroxy group and the excitation spectra of 7-hydroxycoumarins, and exploited that relationship in the design of the ratiometric probes; as a result, most of the synthesized probes showed ratiometric Zn(2+)-sensing properties. Then, we designed and synthesized ratiometric Zn(2+) probes that can be excited with visible light, by choosing adequate substituents on coumarin dyes. Since one of the probes could permeate living cell membranes, we introduced the probe to living RAW264 cells and observed the intracellular Zn(2+) concentration via ratiometric fluorescence microscopy. As a result, the ratio value of the probe changed quickly in response to intracellular Zn(2+) concentration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1520-510X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
17
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7630-8
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Design and synthesis of coumarin-based Zn(2+) probes for ratiometric fluorescence imaging.
pubmed:affiliation
Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't