Linked Life Data

19591244

Source:http://linkedlifedata.com/resource/pubmed/id/19591244

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2009-12-23
pubmed:abstractText
It was found that the fluorescence of Tb(3+)-epinephrine (E) complex can be enhanced by both bovine serum albumin (BSA) and sodium dodecylsulfate (SDS), and stabilized by ascorbic acid (AA). It is considered that the fluorescence enhancement of the Tb(3+)-E-BSA-AA-SDS system originates not only from the hydrophobic microenvironment provided by BSA-SDS, but also from the energy transfer from BSA to Tb(3+) in this system. Therefore, a new fluorescence method for the determination of protein concentrations as low as 1.3 x 10(-9) g mL(-1) BSA is established using Tb(3+)-epinephrine complex as probe. The method has been applied for the determination of BSA and human serum albumin in actual samples, and the results obtained are satisfactory. Compared with other fluorescence methods, this method is simpler and more sensitive for the determination of protein. The mechanism of the fluorescence enhancement of the system is studied in detail.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1522-7243
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
372-8
pubmed:meshHeading
pubmed:articleTitle
Sensitive determination of protein based on the fluorescence enhancement effect of terbium (III)-epinephrine-protein-sodium dodecylsulfate system.
pubmed:affiliation
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't