Linked Life Data

19589363

Source:http://linkedlifedata.com/resource/pubmed/id/19589363

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2009-9-29
pubmed:abstractText
New molecular techniques for allele discrimination such as high-resolution melting (HRM) allow fast, reliable and high-throughput typing without the use of oligonucleotide probes. HRM can also provide an alternative for problematic allele assignments caused by the presence of homopolymeric regions or ambiguous haplotyping. We show here how we have used HRM to introduce upgrades in our KIR allele level typing system for the KIR2DS4, KIR2DL4, and KIR3DL2 genes. The allele assignments obtained by HRM, confirmed by sequencing, were clear-cut and reproducible. This technique allowed for quick and easy upgrading of the KIR allele frequencies and is now validated for future updates of KIR typing. The ongoing refinement of KIR haplotype distribution in our control population will help in disease association studies involving the KIR genes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1879-1166
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
858-63
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Killer cell immunoglobulin-like receptor allele discrimination by high-resolution melting.
pubmed:affiliation
Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, Belfast City Hospital, Belfast, United Kingdom. asensio.gonzalez@belfasttrust.hscni.net
pubmed:publicationType
Journal Article