Source:http://linkedlifedata.com/resource/pubmed/id/19588921
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
2010-12-14
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pubmed:abstractText |
We have elaborated and validated a novel approach for the oriented tethering of proteins such as the epidermal growth factor (EGF) on aminated surfaces. The grafting reactions were optimized to generate a dense and homogeneous EGF layer. Impact of EGF orientation on A-431 cellular response was investigated. Our results demonstrate that, in sharp contrast to responses obtained with soluble EGF supply or with randomly grafted EGF, oriented immobilization of EGF via a de novo designed coiled-coil capture system leads to a sustained phosphorylation of A-431 cell surface EGF receptors. Our results thus indicate that oriented protein immobilization via coiled-coil interactions is an efficient and versatile method to control tethering of bioactive molecules for future applications in the field of regenerative medicine and tissue engineering.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Silanes,
http://linkedlifedata.com/resource/pubmed/chemical/Silicon,
http://linkedlifedata.com/resource/pubmed/chemical/gamma-aminopropyltriethoxysilane
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1520-4812
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
19
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1569-77
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pubmed:meshHeading |
pubmed-meshheading:19588921-Cells, Cultured,
pubmed-meshheading:19588921-Epidermal Growth Factor,
pubmed-meshheading:19588921-Humans,
pubmed-meshheading:19588921-Molecular Structure,
pubmed-meshheading:19588921-Phosphorylation,
pubmed-meshheading:19588921-Receptors, Cell Surface,
pubmed-meshheading:19588921-Silanes,
pubmed-meshheading:19588921-Silicon,
pubmed-meshheading:19588921-Surface Properties
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pubmed:year |
2009
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pubmed:articleTitle |
Epidermal growth factor tethered through coiled-coil interactions induces cell surface receptor phosphorylation.
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pubmed:affiliation |
Department of Chemical Engineering, Groupe de Recherche en Sciences et Technologie Biome?dicales (GRSTB), Ecole Polytechnique de Montre?al, Montre?al (Qc), Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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