Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2010-12-14
pubmed:abstractText
We have elaborated and validated a novel approach for the oriented tethering of proteins such as the epidermal growth factor (EGF) on aminated surfaces. The grafting reactions were optimized to generate a dense and homogeneous EGF layer. Impact of EGF orientation on A-431 cellular response was investigated. Our results demonstrate that, in sharp contrast to responses obtained with soluble EGF supply or with randomly grafted EGF, oriented immobilization of EGF via a de novo designed coiled-coil capture system leads to a sustained phosphorylation of A-431 cell surface EGF receptors. Our results thus indicate that oriented protein immobilization via coiled-coil interactions is an efficient and versatile method to control tethering of bioactive molecules for future applications in the field of regenerative medicine and tissue engineering.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1520-4812
pubmed:author
pubmed:issnType
Electronic
pubmed:day
19
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1569-77
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Epidermal growth factor tethered through coiled-coil interactions induces cell surface receptor phosphorylation.
pubmed:affiliation
Department of Chemical Engineering, Groupe de Recherche en Sciences et Technologie Biome?dicales (GRSTB), Ecole Polytechnique de Montre?al, Montre?al (Qc), Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't