Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2009-7-15
pubmed:abstractText
Pancreatic cancer remains a leading cause of death despite its relatively low incidence. As in many other solid tumors, angiogenesis is critical to the growth and metastasis of this cancer. Through serial in vivo passages in mice, we have developed a highly aggressive variant of human pancreatic cancer cell line XPA-1 which shows more rapid primary tumor growth, faster time to metastasis, and more rapid lethality than the parental cell line. The high-metastatic variant developed a much denser tumor vasculature early during growth within the pancreas. Interestingly, examination of the in vitro growth of this aggressive variant yielded no significant difference from the parental cell line. Real-time PCR evaluation of genes involved in angiogenesis revealed a 24-fold increase in Thrombospondin-1 expression in cells derived from the high-metastatic variant when compared with the parental cell line. These findings provide direct evidence that elevated capability for angiogenesis, mediated by specific changes in gene expression, can lead to a large increase in cancer aggressiveness and resulting metastasis. These findings have important implications for the treatment of metastatic disease.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1538-8514
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1779-86
pubmed:meshHeading
pubmed-meshheading:19584238-Animals, pubmed-meshheading:19584238-Ascites, pubmed-meshheading:19584238-Cell Line, Tumor, pubmed-meshheading:19584238-Cell Proliferation, pubmed-meshheading:19584238-Female, pubmed-meshheading:19584238-Gene Expression Profiling, pubmed-meshheading:19584238-Humans, pubmed-meshheading:19584238-Mice, pubmed-meshheading:19584238-Mice, Nude, pubmed-meshheading:19584238-Mice, Transgenic, pubmed-meshheading:19584238-Neoplasm Metastasis, pubmed-meshheading:19584238-Neovascularization, Pathologic, pubmed-meshheading:19584238-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:19584238-Pancreatic Neoplasms, pubmed-meshheading:19584238-RNA, Messenger, pubmed-meshheading:19584238-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19584238-Thrombospondin 1, pubmed-meshheading:19584238-Up-Regulation, pubmed-meshheading:19584238-Xenograft Model Antitumor Assays
pubmed:year
2009
pubmed:articleTitle
Upregulation of thrombospondin-1 and angiogenesis in an aggressive human pancreatic cancer cell line selected for high metastasis.
pubmed:affiliation
Department of Surgery, University of California, San Diego, CA, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural