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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2009-7-7
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pubmed:abstractText |
In an effort to identify proteomic changes that may be useful for radiation biodosimetry, human cells of hematological origin were treated with ionizing radiation or mock-irradiated and then harvested at different times after treatment. Protein lysates were generated from these cells and evaluated by Western blotting using a panel of 301 commercially available antibodies targeting 161 unique proteins. From this screen, we identified 55 ionizing radiation-responsive proteins, including 14 proteins not previously reported to be radiation-responsive at the protein level. The data from this large-scale screen have been assembled into a public website ( http://labs.fhcrc.org/paulovich/biodose_index.html ) that may be of value to the radiation community both as a source of putative biomarkers for biodosimetry and also as a source of validation data on commercially available antibodies that detect radiation-responsive proteins. Using a panel of candidate radiation biomarkers in human cell lines, we demonstrate the feasibility of assembling a complementary panel of radiation-responsive proteins. Furthermore, we demonstrate the feasibility of using blood cell-based proteomic changes for biodosimetry by demonstrating detection of protein changes in circulating cells after total-body irradiation in a canine model.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-10392961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-11264450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-11763352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-12692264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-12852469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-12859226,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-15070431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-15120963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-15150084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-15374940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-15606315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-15940101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-15971857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-16076753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-16217194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-16774833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-16867976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-16954150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-17050475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-17169946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-17379082,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-18382685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-18667413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-7029603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-7684038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-8090205,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19580490-9552351
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0033-7587
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
549-61
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pubmed:dateRevised |
2011-6-6
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pubmed:meshHeading |
pubmed-meshheading:19580490-Animals,
pubmed-meshheading:19580490-Antibodies,
pubmed-meshheading:19580490-Blotting, Western,
pubmed-meshheading:19580490-Cell Line,
pubmed-meshheading:19580490-Dogs,
pubmed-meshheading:19580490-Humans,
pubmed-meshheading:19580490-Phosphorylation,
pubmed-meshheading:19580490-Proteome,
pubmed-meshheading:19580490-Radiation Dosage,
pubmed-meshheading:19580490-Research Design
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pubmed:year |
2009
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pubmed:articleTitle |
Antibody-based screen for ionizing radiation-dependent changes in the Mammalian proteome for use in biodosimetry.
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pubmed:affiliation |
Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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