Source:http://linkedlifedata.com/resource/pubmed/id/19578774
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2009-7-6
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pubmed:abstractText |
Regulatory T cells (Tregs) play an important role in immunological self-tolerance and protect the host from autoimmune disease. However, in cancer immunity, Tregs might block anti-tumor immune responses. Therefore, the depletion of Tregs using a specific agent that suppresses its function or population, such as an anti-CD25 antibody, could promote anti-tumor immune responses. In the present study, a cytotoxicity assay, enzyme-linked immunosorbent spot (ELISPOT) assay and measuring cytokine secretion, were used to study the efficacy of Treg depletion by anti-CD25 antibody added to a dendritic cell/tumor cell (DC/TC) fusion hybrid vaccine in a murine pancreatic cancer model. All the mice treated with the combined therapy of fusion hybrid vaccine and Treg depletion rejected tumor growth in a challenging test, although the rejection rate was 20% both for mice that received the fusion hybrids alone or Treg depletion alone. In addition, combined therapy showed a significantly improved survival in comparison to other treatment or control groups. The NK cell activity for DC/TC fusion + Treg depletion was significantly higher than that for the other treatment groups. Cytotoxic T lymphocyte (CTL) activity for DC/TC could potentially be enhanced by the addition of Treg depletion therapy. The treatments including DC/TC fusion induced IFN-gamma secreting effector cells in ELISPOT assays. Furthermore, a cytometric beads array assay used to measure cytokine secretion showed that DC/TC fusion + Treg depletion stimulated the highest levels of IFN-gamma Th1/Th2 ratios and Th17. These results demonstrate that Treg depletion combined with DC/TC fusion hybrid vaccine enhanced the efficacy of immunotherapy in pancreatic cancer by activating CTLs and NK cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Il2ra protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-17,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2 Receptor alpha Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1021-335X
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pubmed:author |
pubmed-author:HasegawaHiroshiH,
pubmed-author:HoriYuichiY,
pubmed-author:KamigakiTakashiT,
pubmed-author:KuYonsonY,
pubmed-author:KurodaDaisukeD,
pubmed-author:KurodaYoshikazuY,
pubmed-author:MoriyamaHiroakiH,
pubmed-author:NagataMasaoM,
pubmed-author:YamamotoMasashiM,
pubmed-author:YamashitaKimihiroK
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
337-43
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pubmed:meshHeading |
pubmed-meshheading:19578774-Animals,
pubmed-meshheading:19578774-Cancer Vaccines,
pubmed-meshheading:19578774-Dendritic Cells,
pubmed-meshheading:19578774-Female,
pubmed-meshheading:19578774-Interleukin-10,
pubmed-meshheading:19578774-Interleukin-17,
pubmed-meshheading:19578774-Interleukin-2 Receptor alpha Subunit,
pubmed-meshheading:19578774-Interleukin-6,
pubmed-meshheading:19578774-Killer Cells, Natural,
pubmed-meshheading:19578774-Lymphocyte Depletion,
pubmed-meshheading:19578774-Mice,
pubmed-meshheading:19578774-Mice, Inbred C57BL,
pubmed-meshheading:19578774-Pancreatic Neoplasms,
pubmed-meshheading:19578774-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:19578774-T-Lymphocytes, Regulatory,
pubmed-meshheading:19578774-Th1 Cells,
pubmed-meshheading:19578774-Vaccination
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pubmed:year |
2009
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pubmed:articleTitle |
Enhancement of anti-tumor immunity by high levels of Th1 and Th17 with a combination of dendritic cell fusion hybrids and regulatory T cell depletion in pancreatic cancer.
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pubmed:affiliation |
Department of Surgery, Kobe University Graduate School of Medicine, Hyogo 650-0017, Japan.
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pubmed:publicationType |
Journal Article
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