Linked Life Data

19576895

Source:http://linkedlifedata.com/resource/pubmed/id/19576895

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-11-20
pubmed:abstractText
We have analyzed by qRT-PCR and/or RT-PCR the abundance and degradation rate of maternal mRNAs for nine steroid hormone receptors and their possible replacement by corresponding embryonic transcripts in both ovulated oocytes and embryos of zebrafish collected at 0, 1, 2, 4, 8, 12, 24 and 48 h post-fertilization (hpf). The mRNAs encoded the nuclear receptors for progesterone (pr), androgen (ar), estrogen (er alpha, er beta 1 and er beta 2), glucocorticoids (gr), mineralocorticoids (mr) and the membrane progestin receptor-alpha and beta (mpr alpha and beta). gr mRNA was the most abundant maternal transcript in oocytes and early embryos followed by er beta 2 and ar mRNAs. They declined during the first 8 hpf, being replaced, thereafter, by the embryonic messengers. er beta 1 and mr transcript levels were low until 8 hpf, but increased steadily during embryonic transcription from 24 to 48 hpf. pr transcripts were detectable only in ovulated oocytes and at 24 and 48 hpf. At these stages, there was a slight increase of er alpha mRNA that initially was very low. mPr alpha and beta mRNAs were expressed in ovulated oocytes and faintly persisted during the first 4 hpf. There was no subsequent embryonic expression of these transcripts. The possible involvement of maternal mRNAs for glucocorticoid and sex hormone receptors in the programming of early zebrafish development is intriguing, since they mainly occur at stages in which gene replication predominates over transcription.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1095-6840
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
165
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
215-20
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Expression analysis of steroid hormone receptor mRNAs during zebrafish embryogenesis.
pubmed:affiliation
Comparative Endocrinology Laboratory, Department of Biology, University of Padova, Via U. Bassi 58/B, 35131 Padova, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't