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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2009-7-16
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pubmed:abstractText |
Antiviral immunity is triggered by immunorecognition of viral nucleic acids. The cytosolic helicase RIG-I is a key sensor of viral infections and is activated by RNA containing a triphosphate at the 5' end. The exact structure of RNA activating RIG-I remains controversial. Here, we established a chemical approach for 5' triphosphate oligoribonucleotide synthesis and found that synthetic single-stranded 5' triphosphate oligoribonucleotides were unable to bind and activate RIG-I. Conversely, the addition of the synthetic complementary strand resulted in optimal binding and activation of RIG-I. Short double-strand conformation with base pairing of the nucleoside carrying the 5' triphosphate was required. RIG-I activation was impaired by a 3' overhang at the 5' triphosphate end. These results define the structure of RNA for full RIG-I activation and explain how RIG-I detects negative-strand RNA viruses that lack long double-stranded RNA but do contain blunt short double-stranded 5' triphosphate RNA in the panhandle region of their single-stranded genome.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-10786847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-11585785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-11679672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-11805321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-11907320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-1318785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-1357546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-14990954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-15208624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-15231541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-16625202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-16641297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-16648842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-16714379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-17038589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-17038590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-17356690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-17572678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-17804509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-17942531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-18048689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-18158127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-18208841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-18242112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-18243112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-18272355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-18342006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-18446221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-18591409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-19160512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-19604485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-7518923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-7534310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19576794-9486653
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DDX58 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DEAD-box RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/Ddx58 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Oligoribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Polyphosphates,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Double-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/triphosphoric acid
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1097-4180
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pubmed:author |
pubmed-author:AkiraShizuoS,
pubmed-author:BarchetWinfriedW,
pubmed-author:CochChristophC,
pubmed-author:FitzgeraldKatherine AKA,
pubmed-author:HagmannCristina AmparoCA,
pubmed-author:HartmannGuntherG,
pubmed-author:HornungVeitV,
pubmed-author:JankeMarkusM,
pubmed-author:JuranekStefanS,
pubmed-author:KatoHirokiH,
pubmed-author:KawaiTaroT,
pubmed-author:LatzEickeE,
pubmed-author:LudwigJanosJ,
pubmed-author:MihailovicAleksandraA,
pubmed-author:PoeckHendrikH,
pubmed-author:RothAndreasA,
pubmed-author:SchleeMartinM,
pubmed-author:TakeuchiOsamuO,
pubmed-author:TuschlThomasT,
pubmed-author:WardleGregG,
pubmed-author:WimmenauerVeraV
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pubmed:issnType |
Electronic
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pubmed:day |
17
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25-34
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pubmed:dateRevised |
2011-8-1
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pubmed:meshHeading |
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