Linked Life Data

19576678

Source:http://linkedlifedata.com/resource/pubmed/id/19576678

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2009-8-7
pubmed:abstractText
The circular and linear dichroism (CD and LD) spectral properties of the meso-tetrakis(N-methylpyridinium-4-yl)porphyrin (TMPyP)-DNA complex at a [porphyrin]/[DNA] ratio below 0.015 showed that TMPyP intercalates between DNA base pairs. Contrarily, when cis-bis(N-methylpyridinium-4-yl)porphyrin (BMPyP) is associated with DNA, no CD spectrum was induced and a bisignate LD spectrum was observed. These spectral properties of both the TMPyP and BMPyP were essentially retained when the minor groove of the DNA was saturated with 4',6-diamidino-2-phenylindole (DAPI). The fluorescence of the DNA-bound DAPI was effectively quenched by BMPyP and TMPyP. The quenching by BMPyP can be described through a pure static mechanism while TMPyP quenching produced an upward bending curve in the Stern-Volmer plot. Quenching efficiency was by far greater than predicted by the "sphere of action model", suggesting that the DNA provides some additional processes for an effective energy transfer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1873-4200
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
144
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
38-45
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
DNA-binding geometry dependent energy transfer from 4',6-diamidino-2-phenylindole to cationic porphyrins.
pubmed:affiliation
Department of Chemistry, Yeungnam University, Gyeongsan City, Gyeongbuk, 712-749, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't