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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2009-8-3
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pubmed:abstractText |
Post-translational histone modifications have essential roles in controlling nuclear processes; however, the specific mechanisms regulating these modifications and their combinatorial activities remain elusive. Cyclin-dependent kinase 9 (CDK9) regulates gene expression by phosphorylating transcriptional regulatory proteins, including the RNA polymerase II carboxy-terminal domain. Here, we show that CDK9 activity is essential for maintaining global and gene-associated levels of histone H2B monoubiquitination (H2Bub1). Furthermore, CDK9 activity and H2Bub1 help to maintain correct replication-dependent histone messenger RNA (mRNA) 3'-end processing. CDK9 knockdown consistently resulted in inefficient recognition of the correct mRNA 3'-end cleavage site and led to increased read-through of RNA polymerase II to an alternative downstream polyadenylation signal. Thus, CDK9 acts to integrate phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-11157774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-15034139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-15632065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-15762987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-16307923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-16337599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-16510875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-17079134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-17088385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-17406230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-17499042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-17522673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-18042462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-18079404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-18344985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-18374642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-18457900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-18832071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-18927579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-19013272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-19117995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-2163669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19575011-2576416
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 9,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Dichlororibofuranosylbenzimidazole,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleic Acid Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Piperidines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNF20 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNF40 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Protein Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/flavopiridol
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1469-3178
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
894-900
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pubmed:dateRevised |
2010-9-27
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pubmed:meshHeading |
pubmed-meshheading:19575011-Animals,
pubmed-meshheading:19575011-Blotting, Western,
pubmed-meshheading:19575011-Cell Line,
pubmed-meshheading:19575011-Cell Line, Tumor,
pubmed-meshheading:19575011-Chromatin Immunoprecipitation,
pubmed-meshheading:19575011-Cyclin-Dependent Kinase 9,
pubmed-meshheading:19575011-Dactinomycin,
pubmed-meshheading:19575011-Dichlororibofuranosylbenzimidazole,
pubmed-meshheading:19575011-Flavonoids,
pubmed-meshheading:19575011-Histones,
pubmed-meshheading:19575011-Humans,
pubmed-meshheading:19575011-Mice,
pubmed-meshheading:19575011-Nucleic Acid Synthesis Inhibitors,
pubmed-meshheading:19575011-Piperidines,
pubmed-meshheading:19575011-RNA, Messenger,
pubmed-meshheading:19575011-Transcription, Genetic,
pubmed-meshheading:19575011-Ubiquitin-Protein Ligases,
pubmed-meshheading:19575011-Ubiquitination
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pubmed:year |
2009
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pubmed:articleTitle |
CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3'-end processing.
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pubmed:affiliation |
Department of Molecular Oncology, Göttingen Center for Molecular Biosciences, University of Göttingen, Göttingen, Germany.
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pubmed:publicationType |
Journal Article
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