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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3-4
|
| pubmed:dateCreated |
1991-12-31
|
| pubmed:abstractText |
Experimental parameters for optimization of the growth conditions of Besnoitia besnoiti endozoites in vitro are presented. A combination of Hepes-buffered McCoy and Leibovitz (ML) medium with 10% bovine serum was preferable to Eagle's Minimum Essential Medium (MEM) based on either Hank's or Earle's salts. The ML medium and a CO2 balanced gas phase significantly increased the growth rate of the parasite. Infection of Vero cells at 1 h after subcultivation resulted in higher yields of endozoites than infection of 24- or 48-h-old cells. Increased numbers of parasites (6 x 10(7) and 9 x 10(7)) in the infection inoculum per Roux bottle containing Vero cells produced poor yields, while infection with 2 x 10(7) parasites resulted in a 250 to 300-fold increase after 6 days of cultivation with one medium change.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0304-4017
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
39
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
207-13
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading | |
| pubmed:year |
1991
|
| pubmed:articleTitle |
Besnoitia besnoiti: quantitative in vitro studies.
|
| pubmed:affiliation |
Division of Parasitology, Kimron Veterinary Institute, Bet-Dagan, Israel.
|
| pubmed:publicationType |
Journal Article
|