Linked Life Data

19563767

Source:http://linkedlifedata.com/resource/pubmed/id/19563767

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-8-17
pubmed:abstractText
We have developed an ultrasensitive analytical method based on surface-enhanced Raman spectroscopy (SERS) exploiting a Raman probe covalently bound to gold nanoparticles. The biological marker to be detected was adsorbed on functionalized gold nanoparticles. The capture of these nanoparticles via a biorecognition process between the marker and the immobilized receptor was demonstrated by atomic force microscopy (AFM) imaging. The vibrational fingerprints of the Raman probe on the capture substrate were followed to reveal the presence of the biological marker. The method, which was applied to reveal thrombin captured on a substrate containing antithrombin and heparin, resulted in the ability to detect marker concentrations down to the picomolar (pM) level.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1096-0309
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
393
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-54
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Surface-enhanced Raman spectroscopy combined with atomic force microscopy for ultrasensitive detection of thrombin.
pubmed:affiliation
Biophysics and Nanoscience Centre, CNISM, Facoltá di Scienze, Università della Tuscia, Viterbo, Italy. bizzarri@unitus.it
pubmed:publicationType
Journal Article