Linked Life Data

19558451

Source:http://linkedlifedata.com/resource/pubmed/id/19558451

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-8-11
pubmed:abstractText
Activation of G protein-gated inwardly-rectifying K(+) (GIRK or Kir3) channels by metabotropic gamma-aminobutyric acid (B) (GABA(B)) receptors is an essential signalling pathway controlling neuronal excitability and synaptic transmission in the brain. To investigate the relationship between GIRK channel subunits and GABA(B) receptors in cerebellar Purkinje cells at post- and pre-synaptic sites, we used biochemical, functional and immunohistochemical techniques. Co-immunoprecipitation analysis demonstrated that GIRK subunits are co-assembled with GABA(B) receptors in the cerebellum. Immunoelectron microscopy showed that the subunit composition of GIRK channels in Purkinje cell spines is compartment-dependent. Thus, at extrasynaptic sites GIRK channels are formed by GIRK1/GIRK2/GIRK3, post-synaptic densities contain GIRK2/GIRK3 and dendritic shafts contain GIRK1/GIRK3. The post-synaptic association of GIRK subunits with GABA(B) receptors in Purkinje cells is supported by the subcellular regulation of the ion channel and the receptor in mutant mice. At pre-synaptic sites, GIRK channels localized to parallel fibre terminals are formed by GIRK1/GIRK2/GIRK3 and co-localize with GABA(B) receptors. Consistent with this morphological evidence we demonstrate their functional interaction at axon terminals in the cerebellum by showing that GIRK channels play a role in the inhibition of glutamate release by GABA(B) receptors. The association of GIRK channels and GABA(B) receptors with excitatory synapses at both post- and pre-synaptic sites indicates their intimate involvement in the modulation of glutamatergic neurotransmission in the cerebellum.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-10627592, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-10956667, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-11278325, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-11498050, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-11825890, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-11879649, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-11891065, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-12040038, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-12213627, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-12374786, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-12692128, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-12837758, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-12923211, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-14657159, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-14745451, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-15637097, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-15814785, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-15829630, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-16339040, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-16553611, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-16624949, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-16701209, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-17122050, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-1716664, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-17828261, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-17965710, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-18088366, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-18397365, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-18523006, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-19094055, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-2679954, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-4071055, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-7546746, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-7604029, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-7626080, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-7702616, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-7877685, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-8604043, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-8642402, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-8645300, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-8758956, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-8774431, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-8789947, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-8929423, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-9010212, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-9023358, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-9331358, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-9342388, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-9429760, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-9459446, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-9547222, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-9844003, http://linkedlifedata.com/resource/pubmed/commentcorrection/19558451-9920664
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1471-4159
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
110
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1363-76
pubmed:dateRevised
2010-12-3
pubmed:meshHeading
More...