Linked Life Data

19558232

Source:http://linkedlifedata.com/resource/pubmed/id/19558232

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-6-29
pubmed:abstractText
Toxicological investigations on nanoparticles require a comprehensive physico-chemical characterization to get useful information about the powder as well as the behavior of the suspended nanoparticles in water and physiological media. Therefore, we characterized the often used TiO(2) P25 and developed procedures to get stable, homogeneous, and well-defined nanoparticle suspensions. A titration of the zeta potential as a function of the pH yielded the conclusion that the TiO(2) suspension is stable at a pH of 4 or lower. In this region the zeta potential is higher than 30 mV, which guarantees a high stability of the suspended particles. Hence, a stable TiO(2) initial suspension was prepared in 0.1 mM HCl having a mean particle size of 170 +/- 5 nm, which was determined by dynamic light scattering. Furthermore, the initial suspension was added to different physiological media (0.9% NaCl solution, phosphate-buffered saline [PBS], Hanks balanced salt solution [HBSS], Dulbecco's modified Eagle's medium [DMEM]) for studying the agglomeration behavior. As a result, the agglomeration kinetics at the same TiO(2) concentration is independent of the used media. Investigations with PBS containing bovine serum albumin (BSA) and DMEM supplemented with 10% FBS revealed that these protein additions inhibit the agglomeration of the particles. Thus, the physiological media contains particles that are stabilized through the steric or electrosteric effect of BSA and of the proteins in FBS, respectively. Consequently, the particles keep their size from the initial suspension. Finally, our procedure demonstrated on TiO(2) P25 results in homogeneously suspended particles in physiological media. This definite status of the particles means an improvement for toxicological testing and understanding.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1091-7691
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
21 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-9
pubmed:dateRevised
2010-2-23
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Physico-chemical characterization in the light of toxicological effects.
pubmed:affiliation
Fraunhofer Institute for Ceramic Technologies and Systems, Dresden, Germany. tobias.meissner@ikts.fraunhofer.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't