Source:http://linkedlifedata.com/resource/pubmed/id/19549558
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2009-7-22
|
| pubmed:abstractText |
Transplantation of pluripotent adipose stem/stromal cells (ASC) alleviates tissue damage and improves functional deficits in both stroke and cardiovascular disease animal models. Recent studies indicate that the primary mechanism of ASC-induced repair may not be directly related to tissue regeneration through differentiation, but rather through paracrine mechanisms provided by secreted pro-survival and repair-inducing trophic factors. In this study, we have found that ASC-conditioned medium (ASC-CM) potently protected cerebellar granule neurons (CGN) from apoptosis induced by serum and potassium deprivation. Neural cell protection was mostly attributable to activated caspase-3 and Akt-mediated neuroprotective pathway signaling. Specific neutralization of neurotrophic factor activity demonstrated that serum and potassium deprivation-induced Akt-mediated neuroprotection and caspase-3-dependent apoptosis were mainly modulated by IGF-1. These data suggest that of the many neuroprotective factors secreted by ASC, IGF-1 is the major factor that mediates protection against serum and potassium deprivation-induced CGN apoptosis. This study establishes a mechanistic basis supporting the therapeutic application of ASC for neurological disorders, specifically through paracrine support provided by trophic factor secretion.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Casp3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
1872-7972
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| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:day |
2
|
| pubmed:volume |
462
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
76-9
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:19549558-Adipose Tissue,
pubmed-meshheading:19549558-Animals,
pubmed-meshheading:19549558-Apoptosis,
pubmed-meshheading:19549558-Caspase 3,
pubmed-meshheading:19549558-Cell Survival,
pubmed-meshheading:19549558-Cells, Cultured,
pubmed-meshheading:19549558-Cerebellum,
pubmed-meshheading:19549558-Culture Media, Conditioned,
pubmed-meshheading:19549558-Enzyme Activation,
pubmed-meshheading:19549558-Insulin-Like Growth Factor I,
pubmed-meshheading:19549558-Neurons,
pubmed-meshheading:19549558-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:19549558-Potassium,
pubmed-meshheading:19549558-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:19549558-Rats,
pubmed-meshheading:19549558-Rats, Inbred Lew,
pubmed-meshheading:19549558-Signal Transduction,
pubmed-meshheading:19549558-Stromal Cells
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| pubmed:year |
2009
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| pubmed:articleTitle |
Adipose stromal cells-secreted neuroprotective media against neuronal apoptosis.
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| pubmed:affiliation |
Department of Neurology, School of Medicine, Indiana University, 975 W. Walnut St. IB457, Indianapolis, IN 46202, USA.
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| pubmed:publicationType |
Journal Article
|