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pubmed-article:19547713pubmed:abstractTextA simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field. Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1, 4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.lld:pubmed
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pubmed-article:19547713pubmed:articleTitleFerromagnetic levan composite: an affinity matrix to purify lectin.lld:pubmed
pubmed-article:19547713pubmed:affiliationLaboratório de Glicoproteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.lld:pubmed
pubmed-article:19547713pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19547713pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed