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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2009-6-23
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pubmed:abstractText |
A simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field. Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1, 4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-10052113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-11322880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-12618049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-14491328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-14678793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-15026026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-15196647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-15219908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-15281106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-15582555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-16218666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-16716275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-1839122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-18703130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-7107561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-8579345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19547713-9534231
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1110-7251
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pubmed:author |
pubmed-author:AlmeidaFábio C LFC,
pubmed-author:AngeliRenataR,
pubmed-author:AraújoFlávia F BFF,
pubmed-author:CalazansGlícia M TGM,
pubmed-author:CarvalhoLuiz BLBJr,
pubmed-author:CoelhoLuana C B BLC,
pubmed-author:MacielJackeline CJC,
pubmed-author:PaivaPatrícia M GPM,
pubmed-author:SilvaMaria da Paz CMda P,
pubmed-author:ValenteAna PaulaAP,
pubmed-author:da PazNathalia V NNV,
pubmed-author:dos Santos CorreiaMaria TerezaMT
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pubmed:issnType |
Electronic
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pubmed:volume |
2009
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
179106
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:19547713-Canavalia,
pubmed-meshheading:19547713-Chromatography, Liquid,
pubmed-meshheading:19547713-Concanavalin A,
pubmed-meshheading:19547713-Fabaceae,
pubmed-meshheading:19547713-Ferumoxytol,
pubmed-meshheading:19547713-Fructans,
pubmed-meshheading:19547713-Hemagglutination Inhibition Tests,
pubmed-meshheading:19547713-Nuclear Magnetic Resonance, Biomolecular,
pubmed-meshheading:19547713-Plant Lectins,
pubmed-meshheading:19547713-Zymomonas
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pubmed:year |
2009
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pubmed:articleTitle |
Ferromagnetic levan composite: an affinity matrix to purify lectin.
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pubmed:affiliation |
Laboratório de Glicoproteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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