Linked Life Data

19546472

Source:http://linkedlifedata.com/resource/pubmed/id/19546472

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2009-9-21
pubmed:abstractText
To test the hypothesis that a perturbation of endoplasmic reticulum (ER) function is involved in the pathogenesis of osteoarthritis (OA), articular cartilage was isolated from non-OA patients secondary to resection of osteo- or chondrosarcomas. Intra-joint samples of minimal and advanced osteoarthritic cartilage were isolated from patients undergoing total knee arthroplasty and scored for disease severity. Glucose-regulated protein-78 (grp78) and bcl-2-associated athanogene-1 (bag-1) were detected via immunofluorescence as markers of non-homeostatic ER function. Additionally, the expression of type VI collagen and its integrin receptor, NG2, was determined to examine cartilage matrix health and turnover. There was an upregulation of grp78 in advanced OA, and variable expression in minimal OA. Non-OA cartilage was consistently grp78 negative. The downstream regulator bag-1 was also upregulated in OA compared with normal cartilage. Collagen VI was mainly cell-associated in non-OA cartilage, with a more widespread distribution observed in OA cartilage along with increased intracellular staining intensity. The collagen VI integral membrane proteoglycan receptor NG2 was downregulated in advanced OA compared with its patient-matched minimally involved cartilage sample. These results suggest that chondrocytes exhibit ER stress during OA, in association with upregulation of a large secreted molecule, type VI collagen.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1554
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
923-31
pubmed:dateRevised
2010-10-4
pubmed:meshHeading
pubmed-meshheading:19546472-Adult, pubmed-meshheading:19546472-Antigens, pubmed-meshheading:19546472-Biological Markers, pubmed-meshheading:19546472-Bone Neoplasms, pubmed-meshheading:19546472-Cartilage, Articular, pubmed-meshheading:19546472-Chondrosarcoma, pubmed-meshheading:19546472-Collagen Type VI, pubmed-meshheading:19546472-DNA-Binding Proteins, pubmed-meshheading:19546472-Down-Regulation, pubmed-meshheading:19546472-Endoplasmic Reticulum, pubmed-meshheading:19546472-Heat-Shock Proteins, pubmed-meshheading:19546472-Humans, pubmed-meshheading:19546472-Middle Aged, pubmed-meshheading:19546472-Osteoarthritis, Knee, pubmed-meshheading:19546472-Osteosarcoma, pubmed-meshheading:19546472-Proteoglycans, pubmed-meshheading:19546472-Transcription Factors, pubmed-meshheading:19546472-Up-Regulation
pubmed:year
2009
pubmed:articleTitle
Advanced osteoarthritis in humans is associated with altered collagen VI expression and upregulation of ER-stress markers Grp78 and bag-1.
pubmed:affiliation
Northeastern Ohio Universities Colleges of Medicine and Pharmacy, Rootstown, OH 44272, USA. anugent@neoucom.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural