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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2009-6-23
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pubmed:abstractText |
We used a rat model of pancreas cold preservation to assess its effects on islets. Glands were surgically retrieved and stored in University of Wisconsin (UW) solution for 3 hours (Short) or 18 hours (Long) cold ischemia time (CIT). Islet yield was significantly lower in the Long-CIT than the Short-CIT group, as well as islet recovery after overnight culture (P < .01). Islet cell viability after isolation was significantly reduced in the Long-CIT group (P < .05). Reversal of diabetes following transplantation of suboptimal islet grafts occurred earlier in the Short-CIT group than the Long-CIT. All animals in the Short-CIT group and 80% in the Long-CIT group achieved euglycemia. Freshly isolated islets showed a significant increase of JNK and p38 (P < .05) phosphorylation in Long-CIT compared with Short-CIT. Histopathological assessment of the pancreas showed a significantly higher injury score. Proteomic analysis of pancreatic tissue led to identification of 5 proteins consistently differentially expressed between Short-CIT and Long-CIT. Better understanding of the molecular pathways involved in this phenomenon will be of assistance in defining targeted interventions to improve organ use in the clinical arena.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1873-2623
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
41
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1808-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:19545733-Adenosine,
pubmed-meshheading:19545733-Allopurinol,
pubmed-meshheading:19545733-Animals,
pubmed-meshheading:19545733-Cell Survival,
pubmed-meshheading:19545733-Glutathione,
pubmed-meshheading:19545733-Insulin,
pubmed-meshheading:19545733-Ischemia,
pubmed-meshheading:19545733-Islets of Langerhans,
pubmed-meshheading:19545733-Male,
pubmed-meshheading:19545733-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:19545733-Organ Preservation Solutions,
pubmed-meshheading:19545733-Pancreas,
pubmed-meshheading:19545733-Phosphotransferases,
pubmed-meshheading:19545733-Raffinose,
pubmed-meshheading:19545733-Rats,
pubmed-meshheading:19545733-Rats, Inbred Lew,
pubmed-meshheading:19545733-Tissue and Organ Harvesting
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pubmed:year |
2009
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pubmed:articleTitle |
Effects of pancreas cold ischemia on islet function and quality.
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pubmed:affiliation |
Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, Florida, USA. apileggi@med.miami.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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