Linked Life Data

19542117

Source:http://linkedlifedata.com/resource/pubmed/id/19542117

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 7
pubmed:dateCreated
2009-7-6
pubmed:databankReference
pubmed:abstractText
Amplified fragment length polymorphism (AFLP) DNA fingerprinting was investigated as a tool for fast and accurate identification of acetic acid bacteria (AAB) to the species level. One hundred and thirty five reference strains and 15 additional strains, representing 50 recognized species of the family Acetobacteraceae, were subjected to AFLP analysis using the restriction enzyme combination ApaI/TaqI and the primer combination A03/T03. The reference strains had been previously subjected to either DNA-DNA hybridization or 16S-23S rRNA spacer region gene sequence analysis and were regarded as being accurately classified at the species level. The present study revealed that six of these strains should be reclassified, namely Gluconacetobacter europaeus LMG 1518 and Gluconacetobacter xylinus LMG 1510 as Gluconacetobacter xylinus and Gluconacetobacter europaeus, respectively; Gluconacetobacter kombuchae LMG 23726(T) as Gluconacetobacter hansenii; and Acetobacter orleanensis strains LMG 1545, LMG 1592 and LMG 1608 as Acetobacter cerevisiae. Cluster analysis of the AFLP DNA fingerprints of the reference strains revealed one cluster for each species, showing a linkage level below 50 % with other clusters, except for Acetobacter pasteurianus, Acetobacter indonesiensis and Acetobacter cerevisiae. These three species were separated into two, two, and three clusters, respectively. At present, confusion exists regarding the taxonomic status of Gluconacetobacter oboediens and Gluconacetobacter intermedius; the AFLP data from this study supported their classification as separate taxa. The 15 additional strains could all be identified at the species level. AFLP analysis further revealed that some species harboured genetically diverse strains, whereas other species consisted of strains showing similar banding patterns, indicating a more limited genetic diversity. It can be concluded that AFLP DNA fingerprinting is suitable for accurate identification and classification of a broad range of AAB, as well as for the determination of intraspecific genetic diversity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1466-5026
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1771-86
pubmed:meshHeading
pubmed-meshheading:19542117-Acetobacteraceae, pubmed-meshheading:19542117-Amplified Fragment Length Polymorphism Analysis, pubmed-meshheading:19542117-Bacterial Typing Techniques, pubmed-meshheading:19542117-DNA, Bacterial, pubmed-meshheading:19542117-DNA, Ribosomal Spacer, pubmed-meshheading:19542117-DNA Fingerprinting, pubmed-meshheading:19542117-Deoxyribonucleases, Type II Site-Specific, pubmed-meshheading:19542117-Food Microbiology, pubmed-meshheading:19542117-Genes, rRNA, pubmed-meshheading:19542117-Genotype, pubmed-meshheading:19542117-Gluconacetobacter, pubmed-meshheading:19542117-Molecular Sequence Data, pubmed-meshheading:19542117-Nucleic Acid Hybridization, pubmed-meshheading:19542117-Phylogeny, pubmed-meshheading:19542117-RNA, Ribosomal, 16S, pubmed-meshheading:19542117-RNA, Ribosomal, 23S, pubmed-meshheading:19542117-Sequence Analysis, DNA, pubmed-meshheading:19542117-Species Specificity
pubmed:year
2009
pubmed:articleTitle
Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii.
pubmed:affiliation
BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium. ilse.cleenwerck@ugent.be
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies