Source:http://linkedlifedata.com/resource/pubmed/id/19541820
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
14
|
pubmed:dateCreated |
2009-10-2
|
pubmed:abstractText |
In essential thrombocythemia (ET), the JAK2-V617F mutation is usually restricted to a subpopulation of neutrophils and platelets, and production of JAK2 wild-type (WT) platelets is not suppressed. Nonmutated precursor cells may, therefore, be susceptible to the acquisition of further JAK2 mutations. We used a common single nucleotide polymorphism (SNP) in the JAK2 coding sequence to genotype V617F alleles obtained either by allele-specific restriction enzyme digestion (RED) or by cloning. Both SNP alleles were detected in JAK2 mutant-positive alleles from neutrophils of 10 of 11 ET patients studied using RED compared with 0 of 5 with polycythemia vera. These results were confirmed in cloned products from 5 ET patients and indicate the occurrence of at least 2 separate JAK2 mutation events in the majority of ET patients investigated. In a further ET patient, JAK2 mutant-positive erythroid colonies with either X-allele inactivated were detected, demonstrating they could not have arisen from a common clonal precursor. These results indicate that at least 2 independent JAK2-V617F events occur commonly in ET patients, and they may arise on a polyclonal background. The presence of a JAK2 mutation in ET patients should not, therefore, be equated with a malignant disease.
|
pubmed:grant | |
pubmed:commentsCorrections | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
|
pubmed:issn |
1528-0020
|
pubmed:author | |
pubmed:issnType |
Electronic
|
pubmed:day |
1
|
pubmed:volume |
114
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
3018-23
|
pubmed:meshHeading |
pubmed-meshheading:19541820-Alleles,
pubmed-meshheading:19541820-Erythroid Precursor Cells,
pubmed-meshheading:19541820-Genotype,
pubmed-meshheading:19541820-Humans,
pubmed-meshheading:19541820-Janus Kinase 2,
pubmed-meshheading:19541820-Mutation,
pubmed-meshheading:19541820-Neutrophils,
pubmed-meshheading:19541820-Phenotype,
pubmed-meshheading:19541820-Polymorphism, Single Nucleotide,
pubmed-meshheading:19541820-Thrombocythemia, Essential
|
pubmed:year |
2009
|
pubmed:articleTitle |
In essential thrombocythemia, multiple JAK2-V617F clones are present in most mutant-positive patients: a new disease paradigm.
|
pubmed:affiliation |
Department of Haematology, UCL Cancer Institute, London, United Kingdom.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|