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rdf:type |
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lifeskim:mentions |
umls-concept:C0001128,
umls-concept:C0012935,
umls-concept:C0040624,
umls-concept:C0079419,
umls-concept:C1176299,
umls-concept:C1328695,
umls-concept:C1415927,
umls-concept:C1514562,
umls-concept:C1704675,
umls-concept:C1709390,
umls-concept:C1879547,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
32
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pubmed:dateCreated |
2009-8-3
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pubmed:abstractText |
The tumor suppressor p53 regulates cell cycle arrest and apoptosis by transactivating several genes that are critical for these processes. The transcriptional activity of p53 is often regulated by post-translational modifications and its interactions with various transcriptional coactivators. Here we report a physical interaction between the N-terminal transactivation domain (TAD) of p53 and the C-terminal DNA-binding domain of positive cofactor 4 (PC4(CTD)). Using NMR spectroscopy, we showed that residues 35-57 (TAD2) interact with PC4. (15)N,(1)H HSQC and fluorescence competition experiments indicated that TAD binds to the DNA-binding site of PC4. Hepta-phosphorylation of the TAD peptide increased its binding affinity. Computer modeling of the p53N-PC4 complex revealed several important interactions that are reminiscent of those in the single-stranded DNA-PC4 complex. The ubiquitous nature of the acidic transactivation domain of p53 in mediating interactions with several transcription cofactors is also manifested as a DNA mimetic.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-10327062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-10449714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-10518217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-11099028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-11360197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-11514548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-11771996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-12381304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-14597399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-14966284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-15510160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-15654739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-15824059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-15826952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-16122968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-16234232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-16415882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-16432196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-16545103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-16605275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-16793544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-17438265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-17785449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-17803234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-18391200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-18410249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-1846049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-18630911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-18812399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-19066201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-19363523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-2142753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-7881273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-8019132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-8062391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-8381535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-8703021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-8875929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-8972191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-8990123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-9215639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-9271577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-9299341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-9472015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-9482861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-9512709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-9632764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-9724646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-9890972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19525231-9920920
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
284
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
21728-37
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pubmed:dateRevised |
2010-9-27
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pubmed:meshHeading |
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