| pubmed-article:19521018 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19521018 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:19521018 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:19521018 | lifeskim:mentions | umls-concept:C0596508 | lld:lifeskim |
| pubmed-article:19521018 | lifeskim:mentions | umls-concept:C1522564 | lld:lifeskim |
| pubmed-article:19521018 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:19521018 | lifeskim:mentions | umls-concept:C0205359 | lld:lifeskim |
| pubmed-article:19521018 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:19521018 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:19521018 | pubmed:dateCreated | 2009-8-11 | lld:pubmed |
| pubmed-article:19521018 | pubmed:abstractText | BACKGROUND: MicroRNAs (miRNAs) regulate various biological processes through inhibiting the translation of RNA transcripts. Although miRNA-1 (miR-1) and miRNA-133 (miR-133) are abundantly expressed in the adult heart and involved in cardiac hypertrophy, the roles of these miRNAs in spontaneous myocardial differentiation are unknown. METHODS AND RESULTS: The levels of miR-1 and miR-133 in mouse embryonic stem (ES) cells were increased during spontaneous differentiation by 2-dimensional culture, but reduced during forced myocardial differentiation by a histone deacetylase inhibitor, trichostatin A. The overexpression of miR-1 or miR-133 by lentiviral infection reduced the expression of a cardiac-specific gene, Nkx2.5, during differentiation of ES cells. In addition, miR-1 also inhibited alpha-myosin heavy chain expression. The results of luciferase assays revealed that miR-1 recognizes and targets the 3' untranslated region of cyclin-dependent kinase-9 (Cdk9) in ES cells. Overexpression of miR-1 decreased the protein amounts of Cdk9 without affecting the mRNA levels, indicating that miR-1 post-transcriptionally inhibits Cdk9 translation. CONCLUSIOns: miR-1 and miR-133 may play significant roles in the myocardial differentiation of mouse ES cells, and Cdk9 may be involved in this process as a target of miR-1. | lld:pubmed |
| pubmed-article:19521018 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19521018 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19521018 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19521018 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:19521018 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19521018 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:19521018 | pubmed:issn | 1347-4820 | lld:pubmed |
| pubmed-article:19521018 | pubmed:author | pubmed-author:HasegawaKojiK | lld:pubmed |
| pubmed-article:19521018 | pubmed:author | pubmed-author:WadaHiromichi... | lld:pubmed |
| pubmed-article:19521018 | pubmed:author | pubmed-author:MorimotoTatsu... | lld:pubmed |
| pubmed-article:19521018 | pubmed:author | pubmed-author:KawamuraTeruh... | lld:pubmed |
| pubmed-article:19521018 | pubmed:author | pubmed-author:KitaToruT | lld:pubmed |
| pubmed-article:19521018 | pubmed:author | pubmed-author:YouW TWT | lld:pubmed |
| pubmed-article:19521018 | pubmed:author | pubmed-author:ShimatsuAkira... | lld:pubmed |
| pubmed-article:19521018 | pubmed:author | pubmed-author:TakanabeRieko... | lld:pubmed |
| pubmed-article:19521018 | pubmed:author | pubmed-author:TakayaTomohid... | lld:pubmed |
| pubmed-article:19521018 | pubmed:author | pubmed-author:KaichiShinjiS | lld:pubmed |
| pubmed-article:19521018 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19521018 | pubmed:volume | 73 | lld:pubmed |
| pubmed-article:19521018 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19521018 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19521018 | pubmed:pagination | 1492-7 | lld:pubmed |
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| pubmed-article:19521018 | pubmed:meshHeading | pubmed-meshheading:19521018... | lld:pubmed |
| pubmed-article:19521018 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19521018 | pubmed:articleTitle | MicroRNA-1 and MicroRNA-133 in spontaneous myocardial differentiation of mouse embryonic stem cells. | lld:pubmed |
| pubmed-article:19521018 | pubmed:affiliation | Division of Translational Research, Kyoto Medical Center, National Hospital Organization, Kyoto, Japan. | lld:pubmed |
| pubmed-article:19521018 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19521018 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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