Linked Life Data

19521018

Source:http://linkedlifedata.com/resource/pubmed/id/19521018

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2009-8-11
pubmed:abstractText
BACKGROUND: MicroRNAs (miRNAs) regulate various biological processes through inhibiting the translation of RNA transcripts. Although miRNA-1 (miR-1) and miRNA-133 (miR-133) are abundantly expressed in the adult heart and involved in cardiac hypertrophy, the roles of these miRNAs in spontaneous myocardial differentiation are unknown. METHODS AND RESULTS: The levels of miR-1 and miR-133 in mouse embryonic stem (ES) cells were increased during spontaneous differentiation by 2-dimensional culture, but reduced during forced myocardial differentiation by a histone deacetylase inhibitor, trichostatin A. The overexpression of miR-1 or miR-133 by lentiviral infection reduced the expression of a cardiac-specific gene, Nkx2.5, during differentiation of ES cells. In addition, miR-1 also inhibited alpha-myosin heavy chain expression. The results of luciferase assays revealed that miR-1 recognizes and targets the 3' untranslated region of cyclin-dependent kinase-9 (Cdk9) in ES cells. Overexpression of miR-1 decreased the protein amounts of Cdk9 without affecting the mRNA levels, indicating that miR-1 post-transcriptionally inhibits Cdk9 translation. CONCLUSIOns: miR-1 and miR-133 may play significant roles in the myocardial differentiation of mouse ES cells, and Cdk9 may be involved in this process as a target of miR-1.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Cdk9 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 9, http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Hydroxamic Acids, http://linkedlifedata.com/resource/pubmed/chemical/MicroRNAs, http://linkedlifedata.com/resource/pubmed/chemical/Mirn1 microRNA, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Mirn133 microRNA, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Myosin Heavy Chains, http://linkedlifedata.com/resource/pubmed/chemical/Nkx2-5 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/myosin heavy chain-1, mouse, http://linkedlifedata.com/resource/pubmed/chemical/trichostatin A
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1347-4820
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1492-7
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
MicroRNA-1 and MicroRNA-133 in spontaneous myocardial differentiation of mouse embryonic stem cells.
pubmed:affiliation
Division of Translational Research, Kyoto Medical Center, National Hospital Organization, Kyoto, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't