Linked Life Data

1951698

Source:http://linkedlifedata.com/resource/pubmed/id/1951698

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5 Pt 1
pubmed:dateCreated
1991-12-20
pubmed:abstractText
Gastrointestinal processing and absorption in vivo of 125I-labeled epidermal growth factor (125I-EGF) was studied in isolated stomach, jejunum, and ileum of 12-day-old suckling rats. Dose dependency and regional differences in the processing and absorption of EGF were determined. At 60 min after administration of 125I-EGF into the isolated gastric lumen, greater than 90% of radioactive material was recovered from gastric wall and lumen in both suckling and weanling rats. On the other hand, a considerable amount of EGF was absorbed (in immunoreactive and receptor active form) from isolated jejunum and ileum of suckling rats. The absorption of EGF from isolated jejunum and ileum increased linearly with the dose of EGF administered up to 1 microgram/rat; absorption of orogastrically administered EGF was also increased linearly with the dose of EGF administered (up to 5 micrograms/rat). Reverse-phase high-performance liquid chromatographic analysis of tissue extracts demonstrated that 125I-EGF is stable in gastric lumen but only partially stable in the jejunal lumen. Carboxy-terminally processed forms of EGF were detected in the luminal flushings of jejunum and ileum and in the wall of the stomach, jejunum, and ileum. 125I-des(48-53)EGF was found in ileal flushings and in the walls of the stomach, jejunum, and ileum, whereas 125I-des(53)EGF and 125I-des(49-53)EGF were detected in jejunal flushings. Receptor binding of 125I-des(53)EGF was significantly greater (by 57%) than that of intact 125I-EGF, but receptor binding of 125I-des(49-53)EGF and 125I-des(48-53)EGF was greatly diminished by 57 and 86%, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
261
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
G790-8
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Regional differences in gastrointestinal processing and absorption of epidermal growth factor in suckling rats.
pubmed:affiliation
Department of Pediatrics, University of Arizona, College of Medicine, Tucson 85724.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.