Linked Life Data

19514920

Source:http://linkedlifedata.com/resource/pubmed/id/19514920

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-6-11
pubmed:abstractText
The Organisation for Economic Co-operation and Development (OECD) Test Guideline 429 for the local lymph node assay (LLNA) indicates a minimum of 4 mice per dose group, or of 5 mice if statistics are required. Recent discussions at the Interagency Coordinating Committee for the Validation of Alternative Methods (ICCVAM) have led to suggestions that there should be a change to LLNA protocol requirements to mandate a minimum of 5 mice per group. Although it is not certain that any such proposal will be made, the debate is an important one and prompts reconsideration of animal requirements in the LLNA. In this paper we have conducted an analysis of published data from our own laboratories to determine whether the use of 4 or of 5 mice has had any practical impact on the outcome of the assay. Of the data sets for 17 chemicals in the 4-animal assay (14 positive, 1 uncertain, and 2 negative), 16 results were identical in the 5-animal assay. A marginally positive result in the 4-animal assay was negative in the 5-animal assay. Where potency determinations were made, the outcomes were essentially identical in the 2 forms of the LLNA. Consequently, it is concluded that there is no scientific justification for removing the option to use a 4-animal version of the LLNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1556-9535
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19-22
pubmed:dateRevised
2009-11-17
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
The impact of LLNA group size on the identification and potency classification of skin sensitizers: a review of published data.
pubmed:affiliation
DABMEB Consultancy Ltd, Sharnbrook, UK. david.basketter@ukonline.co.uk
pubmed:publicationType
Journal Article, Review