| pubmed-article:19513614 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19513614 | lifeskim:mentions | umls-concept:C0032136 | lld:lifeskim |
| pubmed-article:19513614 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:19513614 | lifeskim:mentions | umls-concept:C0040048 | lld:lifeskim |
| pubmed-article:19513614 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:19513614 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:19513614 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:19513614 | lifeskim:mentions | umls-concept:C1527177 | lld:lifeskim |
| pubmed-article:19513614 | lifeskim:mentions | umls-concept:C0441513 | lld:lifeskim |
| pubmed-article:19513614 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:19513614 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:19513614 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:19513614 | pubmed:dateCreated | 2009-6-10 | lld:pubmed |
| pubmed-article:19513614 | pubmed:abstractText | SARS coronavirus (SARS-CoV) is the etiologic agent of severe acute respiratory syndrome. The aim of this study was to construct Sars-CoV membrane (M), nucleocapsid (N) and spike 2 (S2) gene eukaryotic expression plasmids, and identify their expression in vitro. Gene fragments encoding N protein, M protein and S2 protein of SARS-CoV were amplified by PCR using cDNA obtained from lung samples of SARS patients as template, and subcloned into pcDNA3.1 vector to form eukaryotic expression plasmids. SARS-CoV protein eukaryotic expression plasmids were transfected respectively into CHO cells. Immunohistochemistry was employed to detect the expression of the structural proteins of SARS-CoV in transfected cells. SARS-CoV protein eukaryotic expression plasmids were successfully constructed by identification with digestion of restriction enzymes and sequencing. M, N and S2 proteins of SARS-CoV were detected in the cytoplasm of transfected CHO cells. It was concluded that these recombinant eukaryotic expression plasmids were constructed successfully, and SARS-CoV encoding proteins could activate transcription and expression of hfgl2 gene. | lld:pubmed |
| pubmed-article:19513614 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19513614 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19513614 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19513614 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19513614 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19513614 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19513614 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19513614 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19513614 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19513614 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19513614 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19513614 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19513614 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19513614 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19513614 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19513614 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:19513614 | pubmed:issn | 1672-0733 | lld:pubmed |
| pubmed-article:19513614 | pubmed:author | pubmed-author:DannC ECE | lld:pubmed |
| pubmed-article:19513614 | pubmed:author | pubmed-author:HouJinlinJ | lld:pubmed |
| pubmed-article:19513614 | pubmed:author | pubmed-author:LuoXiaopingX | lld:pubmed |
| pubmed-article:19513614 | pubmed:author | pubmed-author:MODIEE | lld:pubmed |
| pubmed-article:19513614 | pubmed:author | pubmed-author:WangZhanhuiZ | lld:pubmed |
| pubmed-article:19513614 | pubmed:author | pubmed-author:YanWeimingW | lld:pubmed |
| pubmed-article:19513614 | pubmed:author | pubmed-author:WangHongwuH | lld:pubmed |
| pubmed-article:19513614 | pubmed:author | pubmed-author:HanMeifangM | lld:pubmed |
| pubmed-article:19513614 | pubmed:author | pubmed-author:YaoHuaningH | lld:pubmed |
| pubmed-article:19513614 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:19513614 | pubmed:volume | 29 | lld:pubmed |
| pubmed-article:19513614 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19513614 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19513614 | pubmed:pagination | 318-23 | lld:pubmed |
| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
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| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
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| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
| pubmed-article:19513614 | pubmed:meshHeading | pubmed-meshheading:19513614... | lld:pubmed |
| pubmed-article:19513614 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19513614 | pubmed:articleTitle | Construction of plasmids expressing Sars-CoV encoding proteins and their effects on transcription of hfgl2 prothrombinase. | lld:pubmed |
| pubmed-article:19513614 | pubmed:affiliation | Department of Infectious Disease, Huazhong University of Science and Technology, Wuhan, 430030, China. hongwuwang@126.com | lld:pubmed |
| pubmed-article:19513614 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19513614 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
