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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2009-6-10
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pubmed:abstractText |
SARS coronavirus (SARS-CoV) is the etiologic agent of severe acute respiratory syndrome. The aim of this study was to construct Sars-CoV membrane (M), nucleocapsid (N) and spike 2 (S2) gene eukaryotic expression plasmids, and identify their expression in vitro. Gene fragments encoding N protein, M protein and S2 protein of SARS-CoV were amplified by PCR using cDNA obtained from lung samples of SARS patients as template, and subcloned into pcDNA3.1 vector to form eukaryotic expression plasmids. SARS-CoV protein eukaryotic expression plasmids were transfected respectively into CHO cells. Immunohistochemistry was employed to detect the expression of the structural proteins of SARS-CoV in transfected cells. SARS-CoV protein eukaryotic expression plasmids were successfully constructed by identification with digestion of restriction enzymes and sequencing. M, N and S2 proteins of SARS-CoV were detected in the cytoplasm of transfected CHO cells. It was concluded that these recombinant eukaryotic expression plasmids were constructed successfully, and SARS-CoV encoding proteins could activate transcription and expression of hfgl2 gene.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/FGL2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fibrinogen,
http://linkedlifedata.com/resource/pubmed/chemical/M protein, Coronavirus,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleocapsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thromboplastin,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/nucleocapsid protein, Coronavirus,
http://linkedlifedata.com/resource/pubmed/chemical/spike glycoprotein, coronavirus
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1672-0733
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
318-23
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pubmed:meshHeading |
pubmed-meshheading:19513614-Animals,
pubmed-meshheading:19513614-CHO Cells,
pubmed-meshheading:19513614-Cricetinae,
pubmed-meshheading:19513614-Cricetulus,
pubmed-meshheading:19513614-Fibrinogen,
pubmed-meshheading:19513614-Genetic Vectors,
pubmed-meshheading:19513614-Humans,
pubmed-meshheading:19513614-Membrane Glycoproteins,
pubmed-meshheading:19513614-Nucleocapsid Proteins,
pubmed-meshheading:19513614-Plasmids,
pubmed-meshheading:19513614-Recombinant Proteins,
pubmed-meshheading:19513614-SARS Virus,
pubmed-meshheading:19513614-Severe Acute Respiratory Syndrome,
pubmed-meshheading:19513614-Thromboplastin,
pubmed-meshheading:19513614-Transcription, Genetic,
pubmed-meshheading:19513614-Transfection,
pubmed-meshheading:19513614-Viral Envelope Proteins,
pubmed-meshheading:19513614-Viral Matrix Proteins
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pubmed:year |
2009
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pubmed:articleTitle |
Construction of plasmids expressing Sars-CoV encoding proteins and their effects on transcription of hfgl2 prothrombinase.
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pubmed:affiliation |
Department of Infectious Disease, Huazhong University of Science and Technology, Wuhan, 430030, China. hongwuwang@126.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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